Frederik Andre Hansen

Postdoctoral Fellow - Section for Pharmaceutical Chemistry

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Email f.a.hansen@farmasi.uio.no

Room 222

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Visiting address Sem Sælands vei 3 Farmasibygningen 0371 OSLO

Postal address Postboks 1068 Blindern 0316 Oslo

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Research interests

My research focuses on pharmaceutical analytical chemistry, with special emphasis on sample preparation strategies coupled to liquid chromatography - mass spectrometry (LC-MS) for bioanalytical applications. Green chemistry is central in my research, focusing on miniaturizing the scale of sample preparation and improving the environmentally friendliness of the chemicals used. I also have an interest in multivariate data analysis and its application for chemometry. In more details:

Development of the microextraction technique Electromembrane extraction (EME): extraction chemistry, technical formats, method development strategies, commercialization, theoretical understanding.
Expanding our capabilities for extraction of drugs, endogenous metabolites, and peptides from biological samples.
Developing concepts towards high-throughput implementation.
Application of chemometric approaches to better understand how we should design the chemistry of our extraction systems, and development of models to predict appropriate experimental conditions in order to eliminate the need for empirical optimization.

Skills - how I may be able to help you

Sample preparation - particularly towards bioanalysis
High-throughput and/or sensitive LC-MS method development and operation
Simultaneous analysis of many substances by LC-MS
Design-of-experiments (DOE) methodology and analysis
Multivariate data analysis
Basic machine learning and programming in MATLAB

Teaching activities

Teaching students of pharmacy at UiO at bachelor and master level. Courses include:

Academic background

Postdoc position since 2021, Department of Pharmacy, University of Oslo
PhD in drug analysis. Department of Pharmacy, University of Oslo (2021)
Master of Pharmaceutical Sciences, University of Copenhagen, Denmark (2018)

Awards

PharmaSchool H.C. Ørsted medal as top graduate from School of Pharmaceutical Sciences, University of Copenhagen, Denmark (2018).

External links

https://www.researchgate.net/profile/Frederik-Andre-Hansen
https://www.linkedin.com/in/frederik-andr%C3%A9-hansen-0a449b84/
https://orcid.org/0000-0002-1666-0447

Tags: Analytical chemistry, Sample preparation, Drug analysis, Electromembrane extraction

Fabris, André L.; Hansen, Frederik Andre; Yonamine, Mauricio; Pedersen-Bjergaard, Stig & Øiestad, Elisabeth Leere (2024). An efficient and green method for the analysis of synthetic cathinones in whole blood using 96-well electromembrane extraction and LC-MS/MS. Sustainable Chemistry and Pharmacy. ISSN 2352-5541. 38, p. 1–12. doi: 10.1016/j.scp.2024.101494.
Rye, Torstein Kige; Dowlatshah, Samira; Hansen, Frederik Andre; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2024). Electromembrane extraction of peptides – Looking retrospectively into published studies. Advances in Sample Preparation. ISSN 2772-5820. 9. doi: 10.1016/j.sampre.2024.100106.
Dowlatshah, Samira; Rye, Torstein Kige; Hansen, Frederik Andre; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2024). Parallel electromembrane extraction of peptides with monoterpene and medium-length fatty acid deep eutectic solvents. Analytica Chimica Acta. ISSN 0003-2670. 1297. doi: 10.1016/j.aca.2024.342360.
Song, Chenchen; Zhou, Chen; Hansen, Frederik Andre; Hay, Anne Oldeide & Pedersen-Bjergaard, Stig (2024). Optimization of generic conditions for electromembrane extraction of basic substances of moderate or low polarity. Journal of Separation Science. ISSN 1615-9306. 47(3). doi: 10.1002/jssc.202300801.
Zhou, Chen; Dowlatshah, Samira; Hansen, Frederik Andre & Pedersen-Bjergaard, Stig (2024). Generic conditions for electromembrane extraction of polar bases. Talanta: The International Journal of Pure and Applied Analytical Chemistry. ISSN 0039-9140. 267. doi: 10.1016/j.talanta.2023.125215.
Schüller, Maria; Hansen, Frederik Andre & Pedersen-Bjergaard, Stig (2023). Extraction performance of electromembrane extraction and liquid-phase microextraction in prototype equipment. Journal of Chromatography A. ISSN 0021-9673. 1710. doi: 10.1016/j.chroma.2023.464440.
Dowlatshah, Samira; Hansen, Frederik Andre; Zhou, Chen; Ramos-Payán, María; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2023). Electromembrane extraction of peptides based on hydrogen bond interactions. Analytica Chimica Acta. ISSN 0003-2670. 1275. doi: 10.1016/j.aca.2023.341610. Full text in Research Archive
Zhou, Chen; Dowlatshah, Samira; Hay, Anne Oldeide; Schüller, Maria; Pedersen-Bjergaard, Stig & Hansen, Frederik Andre (2023). Generic Liquid Membranes for Electromembrane Extraction of Bases with Low or Moderate Hydrophilicity. Analytical Chemistry. ISSN 0003-2700. 95(23), p. 8982–8989. doi: 10.1021/acs.analchem.3c01052. Full text in Research Archive
Schüller, Maria; Hansen, Frederik Andre; Skaalvik, Tonje Gottenberg & Pedersen-Bjergaard, Stig (2023). Conductive vial electromembrane extraction – Principles and practical operation. Analytical Science Advances (ASA). ISSN 2628-5452. 4(7-8), p. 236–243. doi: 10.1002/ansa.202200065. Full text in Research Archive
Hay, Anne Oldeide; Hansen, Frederik Andre; Psillakis, Elefteria & Pedersen-Bjergaard, Stig (2022). Liquid-phase microextraction in bioanalysis – How green can it be? Green Analytical Chemistry. ISSN 2772-5774. 3. doi: 10.1016/j.greeac.2022.100028.
Primdahl, Karoline Gangestad; Hansen, Frederik Andre; Solum, Eirik Johansson; Nolsøe, Jens Mortansson Jelstrup & Aursnes, Marius (2022). Introduction to Preparative Chromatography: Description of a Setup with Continuous Detection. Journal of Chemical Education. ISSN 0021-9584. 99(6), p. 2372–2377. doi: 10.1021/acs.jchemed.1c00917.
Hansen, Frederik Andre & Pedersen-Bjergaard, Stig (2022). Electromembrane extraction – looking closer into the liquid membrane. Advances in Sample Preparation. ISSN 2772-5820. 2. doi: 10.1016/j.sampre.2022.100020.
Hay, Anne Oldeide; Trones, Roger; Herfindal, Lars; Skrede, Silje & Hansen, Frederik Andre (2022). Determination of methotrexate and its metabolites in human plasma by electromembrane extraction in conductive vials followed by LC-MS/MS. Advances in Sample Preparation. ISSN 2772-5820. 2. doi: 10.1016/j.sampre.2022.100011.
Hansen, Frederik Andre; Petersen, Nickolaj Jacob; Kutter, Jörg P. & Pedersen-Bjergaard, Stig (2022). Electromembrane extraction in microfluidic formats. Journal of Separation Science. ISSN 1615-9306. 45(1), p. 246–257. doi: 10.1002/jssc.202100603. Full text in Research Archive
Skottvoll, Frøydis Sved; Aizenshtadt, Aleksandra; Hansen, Frederik Andre; Amirola Martinez, Mikel; Andersen, Jannike Mørch & Bogen, Inger Lise [Show all 11 contributors for this article] (2022). Direct Electromembrane Extraction-Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. Analysis & Sensing. ISSN 2629-2742. 2(2). doi: 10.1002/anse.202100051. Full text in Research Archive
Eie, Linda Vårdal; Pedersen-Bjergaard, Stig & Hansen, Frederik Andre (2021). Electromembrane extraction of polar substances – Status and perspectives. Journal of Pharmaceutical and Biomedical Analysis. ISSN 0731-7085. 207. doi: 10.1016/j.jpba.2021.114407. Full text in Research Archive
Rye, Torstein Kige; Martinovic, Gordana; Eie, Linda Vårdal; Hansen, Frederik Andre; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2021). Electromembrane extraction of peptides using deep eutectic solvents as liquid membrane. Analytica Chimica Acta. ISSN 0003-2670. 1175. doi: 10.1016/j.aca.2021.338717. Full text in Research Archive
Hansen, Frederik Andre; Tirandaz, Shima & Pedersen-Bjergaard, Stig (2021). Selectivity and efficiency of electromembrane extraction of polar bases with different liquid membranes—Link to analyte properties. Journal of Separation Science. ISSN 1615-9306. 44(13), p. 2631–2641. doi: 10.1002/jssc.202100167.
Skottvoll, Frøydis Sved; Hansen, Frederik; Harrison, Sean; Boger, Ida Caroline Sneis; Mrsa, Ago & Restan, Magnus Saed [Show all 14 contributors for this article] (2021). Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies. Analytical Chemistry. ISSN 0003-2700. 93, p. 3576–3585. doi: 10.1021/acs.analchem.0c05082. Full text in Research Archive
Eie, Linda Vårdal; Rye, Torstein Kige; Hansen, Frederik; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2021). Electromembrane extraction of peptides and amino acids – status and perspectives. Bioanalysis. ISSN 1757-6180. 13(4), p. 277–289. doi: 10.4155/bio-2020-0285.
Hansen, Frederik & Pedersen-Bjergaard, Stig (2021). Electromembrane extraction of streptomycin from biological fluids. Journal of Chromatography A. ISSN 0021-9673. 1639. doi: 10.1016/j.chroma.2021.461915. Full text in Research Archive
Hansen, Frederik; Santigosa-Murillo, Elia; Payan, Maria Ramos; Munoz, Maria; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction using deep eutectic solvents as the liquid membrane. Analytica Chimica Acta. ISSN 0003-2670. 1143, p. 109–116. doi: 10.1016/j.aca.2020.11.044. Full text in Research Archive
Drobnjak, Milena; Hansen, Frederik; Øiestad, Elisabeth Leere; Løvli, Trond; Trones, Roger & Martinsen, Ørjan Grøttem [Show all 7 contributors for this article] (2020). Electromembrane extraction with vials of conducting polymer. LC GC North America. ISSN 1527-5949. 38(8), p. 435–439.
Hansen, Frederik; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2020). Bioanalysis of pharmaceuticals using liquid-phase microextraction combined with liquid chromatography–mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. ISSN 0731-7085. 189, p. 1–12. doi: 10.1016/j.jpba.2020.113446.
Hansen, Frederik; Jensen, Henrik & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction using sacrificial electrodes. Analytical Chemistry. ISSN 0003-2700. 92(7), p. 5595–5603. doi: 10.1021/acs.analchem.0c00626.
Hansen, Frederik; Jaghl, Mohamad Fadi; Øiestad, Elisabeth Leere; Jensen, Henrik; Pedersen-Bjergaard, Stig & Huang, Chuixiu (2020). Impact of ion balance in electromembrane extraction. Analytica Chimica Acta. ISSN 0003-2670. 1124, p. 129–136. doi: 10.1016/j.aca.2020.05.039.
Hansen, Frederik; Kuban, Pavel; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction of highly polar bases from biological samples – Deeper insight into bis(2-ethylhexyl) phosphate as ionic carrier. Analytica Chimica Acta. ISSN 0003-2670. 1115, p. 23–32. doi: 10.1016/j.aca.2020.04.027.
Hansen, Frederik & Pedersen-Bjergaard, Stig (2019). Emerging extraction strategies in analytical chemistry. Analytical Chemistry. ISSN 0003-2700. 92(1), p. 2–15. doi: 10.1021/acs.analchem.9b04677.

View all works in Cristin

Bull Tuner, Marius; Kogler, Stian; Hansen, Frederik Andre; Pedersen-Bjergaard, Stig; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2023). Exploring electromembrane extraction and LC-MS for studying liver organoid metabolomics.
Bull Tuner, Marius; Kogler, Stian; Hansen, Frederik Andre; Pedersen-Bjergaard, Stig; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2022). Exploring electromembrane extraction for sampling the organoid metabolome.
Skottvoll, Frøydis Sved; Harrison, Sean; Hansen, Frederik; Pedersen-Bjergaard, Stig; Sullivan, Gareth & Bogen, Inger Lise [Show all 8 contributors for this article] (2020). Liver mini-organ analyses using liquid chromatography mass spectrometry. Show summary
Organoids (self-organized multi-cellular tissue derived from e.g. patient-derived stem cells) are emerging in vitro 3D representations of human organ functionality, predicted to have a game-changing impact on drug discovery, disease modeling and personalized medicine [1]. Integrating the organoids in a microfluidic chip (i.e. organoids-on-a-chip) would allow for controllable perfusion of nutrients and manipulation of the organoids by the addition of e.g. drugs [2]. Coupling the organoid-on-a-chip online to liquid chromatography mass spectrometry (LC-MS) would enable real-time measurements of the biochemical processes that occur on-chip. However, limited sample sizes, complex matrices and the presence of low abundant analytes also create analytical challenges [3]. Moreover, the organoids are still at the developmental stage, thus fundamental questions on the functionality of the developed organoids remain to be answered. Liver organoid functionality was assessed by protein characterization of adult liver tissue and liver organoids using nanoLC-MS/MS. The proteins identified from the liver organoids covered 70-84% (1003 proteins) of the liver protein expression from adult liver tissue derived from five patients. The proteins that were shared between the adult liver tissue and liver organoids could also relate to central liver pathways (e.g. amino acid biosynthesis and glycolysis). The sample preparation technique electromembrane extraction (i.e. electrophoretic separation across an oil membrane, EME) has shown to have on-chip analytical potential [4] and was thus used in the study of heroin drug metabolism in liver organoids. Multi-well EME (100 µL solutions) allowed for simple and repeatable monitoring of heroin metabolism kinetics without interference from cell medium components (e.g. albumin). The EME extracts were also compatible with different analytical approaches (LC-MS, nanoLC-MS, capillary electrophoresis-UV). Hence, the hyphenation of EME with nanoLC-MS would be a natural next step. References 1. Method of the Year 2017: Organoids. Nature Methods. Vol. 15 (2018) 1-1. 2. Park, S.E., A. Georgescu, and D. Huh, Organoids-on-a-chip. Science. Vol. 364 (2019) 960. 3. Lin, A., F. Sved Skottvoll, S. Rayner, S. Pedersen-Bjergaard, G. Sullivan, S. Krauss, S. Ray Wilson, and S. Harrison, 3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry. ELECTROPHORESIS. Vol. 0 (2019). 4. Hansen, F.A., D. Sticker, J.P. Kutter, N.J. Petersen, and S. Pedersen-Bjergaard, Nanoliter-Scale Electromembrane Extraction and Enrichment in a Microfluidic Chip. Analytical Chemistry. Vol. 90 (2018) 9322-9329.