Ida Kristin Hegna

• Smith, Veronika; Nilssen, Ida Kristine Bu; Hegna, Ida Kristin; Dalhus, Bjørn; Fagerlund, Annette & Økstad, Ole Andreas (2021). CdgL is a degenerate nucleotide cyclase domain protein affecting flagellin synthesis and motility in Bacillus thuringiensis. Research in Microbiology. ISSN 0923-2508. 172(4-5). doi: 10.1016/j.resmic.2021.103850. Full text in Research Archive
• Attieh, Zaynoun; Mouawad, Carine; Rejasse, Agnès; Jehanno, Isabelle; Perchat, Stéphane & Hegna, Ida Kristin [Show all 10 contributors for this article] (2020). The fliK Gene Is Required for the Resistance of Bacillus thuringiensis to Antimicrobial Peptides and Virulence in Drosophila melanogaster. Frontiers in Microbiology. ISSN 1664-302X. 11. doi: 10.3389/fmicb.2020.611220.
• Smith, Veronika; Josefsen, Malin; Lindbäck, Toril; Hegna, Ida Kristin; Finke, Sarah & Tourasse, Nicolas Jacques [Show all 9 contributors for this article] (2020). MogR is a ubiquitous transcriptional repressor affecting motility, biofilm formation and virulence in Bacillus thuringiensis. Frontiers in Microbiology. ISSN 1664-302X. 11. doi: 10.3389/fmicb.2020.610650. Full text in Research Archive Show summary

  Flagellar motility is considered an important virulence factor in different pathogenic bacteria. In Listeria monocytogenes the transcriptional repressor MogR regulates motility in a temperature-dependent manner, directly repressing flagellar- and chemotaxis genes. The only other bacteria known to carry a mogR homolog are members of the Bacillus cereus group, which includes motile species such as B. cereus and Bacillus thuringiensis as well as the non-motile species Bacillus anthracis, Bacillus mycoides and Bacillus pseudomycoides. Furthermore, the main motility locus in B. cereus group bacteria, carrying the genes for flagellar synthesis, appears to be more closely related to L. monocytogenes than to Bacillus subtilis, which belongs to a separate phylogenetic group of Bacilli and does not carry a mogR ortholog. Here, we show that in B. thuringiensis, MogR overexpression results in non-motile cells devoid of flagella. Global gene expression profiling showed that 110 genes were differentially regulated by MogR overexpression, including flagellar motility genes, but also genes associated with virulence, stress response and biofilm lifestyle. Accordingly, phenotypic assays showed that MogR also affects cytotoxicity and biofilm formation in B. thuringiensis. Overexpression of a MogR variant mutated in two amino acids within the putative DNA binding domain restored phenotypes to those of an empty vector control. In accordance, introduction of these mutations resulted in complete loss in MogR binding to its candidate flagellar locus target site in vitro. In contrast to L. monocytogenes, MogR appears to be regulated in a growth-phase dependent and temperature-independent manner in B. thuringiensis 407. Interestingly, mogR was found to be conserved also in non-motile B. cereus group species such as B. mycoides and B. pseudomycoides, which both carry major gene deletions in the flagellar motility locus and where in B. pseudomycoides mogR is the only gene retained. Furthermore, mogR is expressed in non-motile B. anthracis. Altogether this provides indications of an expanded set of functions for MogR in B. cereus group species, beyond motility regulation. In conclusion, MogR constitutes a novel B. thuringiensis pleiotropic transcriptional regulator, acting as a repressor of motility genes, and affecting the expression of a variety of additional genes involved in biofilm formation and virulence.

• Brudal, Espen; Lampe, Elisabeth Opsjøn; Reubsaet, Leon; Roos, Norbert; Hegna, Ida Kristin & Thrane, Ida Marie [Show all 8 contributors for this article] (2015). Vaccination with outer membrane vesicles from Francisella noatunensis reduces development of francisellosis in a zebrafish model. Fish and Shellfish Immunology. ISSN 1050-4648. 42(1), p. 50–57. doi: 10.1016/j.fsi.2014.10.025.
• Vörös, Aniko; Simm, Roger; Slamti, Leyla; McKay, Matthew J.; Hegna, Ida Kristin & Nielsen-Leroux, Christina [Show all 12 contributors for this article] (2014). SecDF as part of the sec-translocase facilitates efficient secretion of Bacillus cereus toxins and cell wall-associated proteins. PLOS ONE. ISSN 1932-6203. 9:e0103326(8). doi: 10.1371/journal.pone.0103326.
• Rukke, Håkon Valen; Hegna, Ida Kristin & Petersen, Fernanda Cristina (2012). Identification of a functional capsule locus in Streptococcus mitis. Molecular Oral Microbiology (MOM). ISSN 2041-1006. 27(2), p. 95–108. doi: 10.1111/j.2041-1014.2011.00635.x.
• Austarheim, Ingvild; Christensen, Bjørn Erik; Hegna, Ida Kristin; Petersen, Bent O.; Duus, Jens O & Bye, Ragnar [Show all 10 contributors for this article] (2012). Chemical and biological characterization of pectin-like polysaccharides from the bark of the Malian medicinal tree Cola cordifolia. Carbohydrate Polymers. ISSN 0144-8617. 89(1), p. 259–268. doi: 10.1016/j.carbpol.2012.03.005.
• Rukke, Håkon Valen; Hegna, Ida Kristin & Petersen, Fernanda Cristina (2012). Identification of a functional capsule locus in Streptococcus mitis. Molecular Oral Microbiology (MOM). ISSN 2041-1006. 27(2), p. 95–108. doi: 10.1111/j.2041-1014.2011.00635.x.
• Smistad, Gro; Nguyen, Nguyen Binh; Hegna, Ida Kristin & Sande, Sverre Arne (2011). Influence of liposomal formulation variables on the interaction with Candida albicans in biofilm; a multivariate approach. Journal of liposome research. ISSN 0898-2104. 21(1), p. 9–16. doi: 10.3109/08982101003629009.
• Smistad, Gro; Nguyen, Nguyen Binh; Hegna, Ida Kristin & Sande, Sverre Arne (2010). Influence of liposomal formulation variables on the interaction with Candida albicans in biofilm; a multivariate approach. Journal of liposome research. ISSN 0898-2104. doi: 10.3109/08982101003629009.
• Tourasse, Nicolas; Helgason, Erlendur; Økstad, Ole Andreas Løchen; Hegna, Ida Kristin & Kolstø, Anne-Brit (2006). The Bacillus cereus group: novel aspects of population structure and genome dynamics. Journal of Applied Microbiology. ISSN 1364-5072. 101, p. 579–593.
• Risøen, Per Arne; Ronning, P; Hegna, Ida Kristin & Kolstø, Anne-Brit (2004). Characterization of a broad range antimicrobial substance from Bacillus cereus. Journal of Applied Microbiology. ISSN 1364-5072. 96, p. 648–655.
• Hegna, Ida Kristin; Bratland, Hege & Kolstø, Anne-Brit (2001). BceS1, a new addition to the type III restriction and modification family. FEMS Microbiology Letters. ISSN 0378-1097. 202, p. 189–193.
• Kolstø, Anne-Brit; Helgason, Erlendur; Økstad, Ole Andreas Løchen; Caugant, Dominique A; Johansen, Henning A. & Fouet, Agnes [Show all 8 contributors for this article] (2000). Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis - one species on the basis of genetic evidence. Applied and Environmental Microbiology. ISSN 0099-2240. p. 2627–2630.
• Helgason, Erlendur; Økstad, Ole Andreas Løchen; Caugant, Dominique A; Johansen, Henning A.; Fouet, Agnes & Mock, Michele [Show all 8 contributors for this article] (2000). Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis - one species based on genetic evidence. Applied and Environmental Microbiology. ISSN 0099-2240. 66, p. 2627–2630.
• Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Lindbäck, Toril; Rishovd, Anne-Lise & Kolstø, Anne-Brit (1999). Genome organization is not conserved between Bacillus cereus and Bacillus subtilis. Microbiology. ISSN 0026-2617. 145, p. 621–631.
• Kolstø, Anne-Brit; Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Lindbäck, Toril & Rishovd, Anne-Lise (1999). Genome organization is not conserved between Bacillus cereus and Bacillus subtilis. Microbiology. ISSN 0026-2617. p. 621–631.
• Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Lindbäck, Toril; Rishovd, Anne-Lise & Kolstø, Anne-Brit (1999). Genome organisation is not conserved between Bacillus cereus and Bacillus subtilis. Microbiology. ISSN 0026-2617. 145, p. 621–631.
• Helgason, Erlendur; Caugant, Dominique A; Lecadet, M.-M.; Chen, Ying; Mahillon, J. & Løvgren, Ann [Show all 9 contributors for this article] (1998). Genetic diversity of Bacillus cereus / B. thuringiensis isolates from natural sources. Current Microbiology. ISSN 0343-8651. p. 80–87.
• Helgason, Erlendur; Caugant, Dominique A; Lecadet, M.-M.; Chen, Ying; Mahillon, J. & Løvgren, Ann [Show all 9 contributors for this article] (1998). Genetic diversity of Bacillus cereus/B. thuringiensis isolates from natural sources. Current Microbiology. ISSN 0343-8651. 37, p. 80–87.
• Hegna, Ida Kristin; Karlstrøm, E.; Lopez, Rodrigo; Kristiansen, T. & Kolstø, Anne-Brit (1992). A type -III DNA restriction and modification system in Bacillus cereus? Gene. ISSN 0378-1119. 114, p. 149–150.
• Løberg, R.M.; Fægri, A. & Hegna, Ida Kristin (1989). Yeast suspension as soiling matter in desinfectant testing. Microbios. ISSN 0026-2633. p. 157–166.
• Hegna, Ida Kristin & Clausen, Ole Petter Fraas (1988). An investigation of the bactericidal and fungicidal effects of certain desinfectants by use of a capacity test. Microbiology. ISSN 0026-2617. 139, p. 473–483.
• Solberg, Olav & Hegna, Ida Kristin (1979). Microbiological Assay of Pantothenic Acid. Vitamins and Coenzymes, Methods in Enzymology,Part D. p. 201–204.
• Løberg, R.M. & Hegna, Ida Kristin (1979). Comparative studies of two preparations containing clorhexidine and cetylpyridinium chloride. Pharm. Acta Helv.. 54, p. 244–249.
• Hegna, Ida Kristin (1979). Further comparison between disinfectant containing quaternary ammonium and chlorhexidine. Meddelelser fra Norsk Farmaceutisk Selskap. ISSN 0029-1927. 41, p. 169–172.
• Hegna, Ida Kristin; Kardel, Kristin & Kardel, M. (1978). Autoclaving of lubricated dental instuments. Scandinavian Journal of Dental Research. ISSN 0029-845X. 86, p. 130–134.
• Hegna, Ida Kristin (1977). An examination of the effect of three phenolic disinfectats on Mycobacterium tuberculosis. Journal of Applied Bacteriology. ISSN 0021-8847. 43, p. 183–187.
• Hegna, Ida Kristin (1977). A comparative investigation of the bactericidal and fungicidal effects of chlorhexidine and cetylpyridinium chloride. Meddelelser fra Norsk Farmaceutisk Selskap. ISSN 0029-1927. 39, p. 188–196.
• Clausen, Ole Petter Fraas & Hegna, Ida Kristin (1977). Determination of the bactericidal and fungicidal effects of Alkylbenzylammonium chloride and propyleneglycol-b-phenylether, singly and in combinations. Meddelelser fra Norsk Farmaceutisk Selskap. ISSN 0029-1927. 39, p. 197–204.
• Hegna, Ida Kristin (1976). In vitro antibacterial effects of some bisbiguanides on certain bacteria which accur in connection with acne vulgaris. Meddelelser fra Norsk Farmaceutisk Selskap. ISSN 0029-1927. 38, p. 34–39.
• Hegna, Ida Kristin (1976). Antibacterial in vitro effects on Corynebacterium acnes NCTC 737. Meddelelser fra Norsk Farmaceutisk Selskap. ISSN 0029-1927. 38, p. 40–47.
• Hegna, Ida Kristin (1976). Antimicrobial effects of some bis-biguanides on certain bacteria which accur in connection with acne vurgaris. Journal of Pharmacy and Pharmacology (JPP). ISSN 0022-3573. 28, p. 261–262.
• Hegna, Ida Kristin (1976). A comparative investigation of the bactericidal fand fungicidal effects of three phenolic disinfectants. Journal of Applied Bacteriology. ISSN 0021-8847. 43, p. 177–181.
• Solberg, Olav; Hegna, Ida Kristin & Clausen, Ole Petter Fraas (1975). Pediococcus acidilactici NCIB a699, a new test organism for microbiological assaying of pantothenic acid. Journal of Applied Bacteriology. ISSN 0021-8847. 39, p. 119–123.
• Clausen, Ole Petter Fraas; Hegna, Ida Kristin & Solberg, Olav (1975). Agglutination tests as an aid to the classification of Pediococci and Aerococci. Journal of the Institute of Brewing. ISSN 0046-9750. 81, p. 440–443.
• Andersen, Solveig Norheim & Hegna, Ida Kristin (1975). An in vitro study of the bactericidal effects on Escherichia coli of some bisbiguanides in aqueous solutions with various pH values. Meddelelser fra Norsk Farmaceutisk Selskap. ISSN 0029-1927. 37, p. 283–288.
• Clausen, Ole Petter Fraas; Hegna, Ida Kristin; Kjeldaas, Liv; Palmer, L.; Solberg, Olav & Tjade, O. H. (1974). Some further aspects concerning the classification of Aerococci and Pediococci. XIth Conference on the Taxonomy of Bacteria.
• Gaudernack, Gustav; Rugstad, Hans Erik; Hegna, Ida Kristin & Prydz, Hans Peter B (1973). Synthesis of serum proteins by a clonal strain of rat heptoma cells. Experimental Cell Research. ISSN 0014-4827. 77, p. 25–30.

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• Rukke, Håkon Valen; Hegna, Ida Kristin & Petersen, Fernanda Cristina (2011). Functional characterization of the Streptococcus mitis capsule locus.
• Rukke, Håkon Valen; Hegna, Ida Kristin & Petersen, Fernanda Cristina (2011). Capsule expression in Streptococcus mitis.
• Økstad, Ole Andreas Løchen; Tourasse, Nicolas; Hegna, Ida Kristin & Kolstø, Anne-Brit (2007). Functional and comparative genomics in pathogenic Bacillus species.
• Fægri, Karoline; Hegna, Ida Kristin; Økstad, Ole Andreas Løchen & Kolstø, Anne-Brit (2007). Comparative analysis of global gene expression in Bacillus cereus ATCC 14579 and a deletion mutant of a membrane transporter with similarity to tetracycline resistance genes.
• Stalheim, Torunn; Hegna, Ida Kristin; Ballance, Simon; Christensen, Bjørn Erik & Granum, Per Einar (2006). Antimicrobial activity of biopolymers isolated from Sphagnum moss.
• Hegna, Ida Kristin; Jaroszewicz, Ewa & Stabell, Fredrik Bernhard (2006). Atomic Force Microscopy in Microbiology.
• Hegna, Ida Kristin (2006). Imaging of DNA, bacteria and spores by atomic force microscopy.
• Hegna, Ida Kristin (2006). Atomic force microscopy used to visualize cell surface of bacteria.
• Hegna, Ida Kristin (2006). Atomic force microscopy used in the imaging of various aspects related to Bacillus cereus.
• Hegna, Ida Kristin & Jaroszewicz, Ewa (2006). Atomic force microscopy used in imaging of biological samples.
• Risøen, Per Arne; Økstad, Ole Andreas Løchen; Hegna, Ida Kristin & Kolstø, Anne-Brit (2003). Characterization of a gene cluster putatively involved in the production of an antimicrobial substance in Bacillus cereus.
• Hegna, Ida Kristin; Jaroszewicz, Ewa & Kolstø, Anne-Brit (2003). BceS1, the type III Resstriction and Modification System in Bacillus cereus ATCC 10987.
• Hegna, Ida Kristin & Kolstø, Anne-Brit (2002). Is the sefishness of the Bacillus cereus ATTC 10987 strain caused by the BceS1 type III restriction and modification system? Show summary

  Several attempts have been made to transform the Bacillus cereus ATCC 10987 strain, but with no success. The nucleotide sequence of an 11kb chromosomal BglII fragment from this strain revealed two closely adjacent open reading frames organized in an operon, of which the deduced amino acids showed 61.6% identity to the type III restriction and modification (R/M) subunits described in G-negative bacteria. The genes were not identified in any of 32 other B.cereus/thuringiensis strains, and several of these strains have successfully been transformed. The system was designated BceS1 as this endonuclease required ATP and Mg2+ as cofactors like other type III endonucleases. Northern blot analysis revealed an enhanced transcription level when the culture was grown in the presence of foreign DNA, and a cell free extract (CFE) from this culture restricted pUC19, whereas a CFE from the same strain grown in plain medium did not. CFE methylation treated pUC 19 was not restricted by the CFE . The CFE methylated the plasmid and protected it against further restriction of the CFE. Evidence for the restriction sites in pUC 19 are demonstrated, and the recognition sequence of the BceS1 are under investigation. BceS1 is the first chromosomal type III R/M system characterized in a Gram positive bacterium.

• Hegna, Ida Kristin & Kolstø, Anne-Brit (2001). A Gram negative type III restriction and modification system in a Gram positive bacterium?
• Helgason, Erlendur; Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Bergan, Pål Andreas & Kolstø, Anne-Brit (2001). Genetic diversity of the Bacillus cereus group.
• Hegna, Ida Kristin & Kolstø, Anne-Brit (2001). Expression of a type III restriction and modification system in Bacillus cereus.
• Hegna, Ida Kristin (2000). Pilletrilling, legemidler og samfunn. Hva er farmasi og hva jobber farmasøytene med? Fokus på mikrobiologi.
• Kolstø, Anne-Brit; Helgason, Erlendur; Økstad, Ole Andreas Løchen; Johansen, Henning A.; Hegna, Ida Kristin & Caugant, Dominique A (2000). Bacillus Thuringiensis, Bacillus Cereus and Bacillus Anthracis: one species based on genetic evidence.
• Kolstø, Anne-Brit; Helgason, Erlendur; Hegna, Ida Kristin; Johansen, Henning A.; Økstad, Ole Andreas Løchen & Caugant, Dominique A [Show all 8 contributors for this article] (1999). Bacillus Cereus, Bacillus Thuringiensis and Bacillus Anthracis-one species based on Genetic Evidence.
• Helgason, Erlendur; Caugant, Dominique A; Hegna, Ida Kristin; Fouet, Agnes; Mock, Michele & Johansen, H A. [Show all 8 contributors for this article] (1999). Bacillus cereus, Bacillus thuringiensis and Bacillus anthracis - one species based on genetic evidence.
• Helgason, Erlendur; Caugant, Dominique A; Lecadet, M.-M.; Olsen, Ingar; Hegna, Ida Kristin & Kvaløy, Kirsti [Show all 7 contributors for this article] (1999). Genetic diversity and population structure of Bacillus cereus/Bacillus thuringiensis isolates from diverse sources.
• Hegna, Ida Kristin; Bratland, Hege & Kolstø, Anne-Brit (1999). Horizontal gene transfer between Gram positive and Gram negative bacteria?
• Hegna, Ida Kristin; Bratland, Hege & Kolstø, Anne-Brit (1999). Type III restriction and modification enzyme locus in Bacillus cereus?
• Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Lindbäck, Toril; Rishovd, Anne-Lise & Kolstø, Anne-Brit (1999). Genome organization is not conserved between Bacillus cereus and Bacillus subtilis.
• Kolstø, Anne-Brit; Helgason, Erlendur; Caugant, Dominique A; Hegna, Ida Kristin; Fouet, Agnes & Mock, Michele [Show all 8 contributors for this article] (1999). Bacillus anthracis, Bacillus cereus and Bacillus thuringiensis - one species based on genetic evidence.
• Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Lindbäck, Toril; Rishovd, Anne-Lise & Kolstø, Anne-Brit (1999). Genome organization is not conserved between Bacillus cereus and Bacillus subtilis.
• Økstad, Ole Andreas Løchen; Lindbäck, Toril; Hegna, Ida Kristin; Rishovd, Anne-Lise & Kolstø, Anne-Brit (1998). Analysis of 75kb random DNA sequence from Bacillus cereus ATCC 10987.
• Kolstø, Anne-Brit; Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Lindbäck, Toril; Rishovd, Anne-Lise & Helgason, Erlendur [Show all 8 contributors for this article] (1997). Genome Analysis of Bacillus cereus and Bacillus thuringiensis.
• Kolstø, Anne-Brit; Økstad, Ole Andreas Løchen; Hegna, Ida Kristin; Lindbäck, Toril; Rishovd, Anne-Lise & Helgason, Erlendur [Show all 8 contributors for this article] (1997). Genome Analysis of Bacillus cereus and Bacillus thyringiensis.
• Hegna, Ida Kristin (1997). Ny kunnskap om gamle bakterier, Antibiotikabruk på ville veier.
• Hegna, Ida Kristin; Bjørge, S. & Kolstø, Anne-Brit (1997). Analysis of a possible efflux gene in Bacillus cereus.
• Hegna, Ida Kristin; Bjørge, S. & Kolstø, Anne-Brit (1997). Analysis of the chrA gene i Bacillus cereus.
• Økstad, Ole Andreas Løchen; Rishovd, Anne-Lise; Lindbäck, Toril; Hegna, Ida Kristin & Kolstø, Anne-Brit (1997). Random sequence anlysis of a Bacillus cereus genome; codon usage, GC content, and gene organization.
• Helgason, Erlendur; Caugant, Dominique A; Lecadet, M.-M.; Hegna, Ida Kristin; Kvaløy, Kirsti & Kolstø, Anne-Brit (1997). Genetic diversity and population structure of Bacillus cereus/B.thuringiensis isolated from diverse sources.
• Helgason, Erlendur; Cugant, D.A.; Lecadet, M.-M.; Hegna, Ida Kristin; Kvaløy, Kirsti & Kolstø, Anne-Brit (1997). Genetic diversity and population structure of Bacillus cereus/Bacillus thuringiensis isolates from natural sources in Norway.
• Hegna, Ida Kristin; Bjørge, S.; Rishovd, Anne-Lise & Kolstø, Anne-Brit (1997). The chromate resistance gene in Bacillus cereus - a multifunctional gene?
• Kolstø, Anne-Brit; Økstad, Ole Andreas Løchen; Hegna, Ida Kristin & Lindbäck, Toril (1996). Genome analysis of Bacillus cereus/Bacillus thuringiensis strains.
• Hegna, Ida Kristin; Nording, M.; Rishovd, Anne-Lise & Kolstø, Anne-Brit (1996). Diversity of Bacillus cereus.
• Hegna, Ida Kristin; Rishovd, Anne-Lise; Kolstø, Anne-Brit & Wright, Marianne (1994). Chromate resistance in Bacillus cereus ATCC 10987.
• Hegna, Ida Kristin; Lindbäck, Toril; Lopez, Rodrigo; Kristiansen, T. & Kolstø, Anne-Brit (1993). Novel genes in Bacillus cereus.
• Hegna, Ida Kristin & Kolstø, Anne-Brit (1993). Novel genes in Bacillus cereus ATCC10987.
• Hegna, Ida Kristin & Clausen, Ole Petter Fraas (1980). Sårbehandling og Debrisan. Chlorhexidine and Quats. Tidsskrift for Den norske legeforening. ISSN 0029-2001. 100, p. 582–583.
• Hegna, Ida Kristin & Clausen, Ole Petter Fraas (1979). Sårbehandling og Debrisan. Tidsskrift for Den norske legeforening. ISSN 0029-2001. 99.
• Kardel, Kristin; Hegna, Ida Kristin & Kardel, M. (1976). En mikrobiologisk undersøkelse over Mikro Megas E-spray. Den norske tannlegeforenings tidende. ISSN 0029-2303. p. 259–262.

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