Stig Pedersen-Bjergaard

Professor - Section for Pharmaceutical Chemistry

Norwegian version of this page

Email stig.pedersen-bjergaard@farmasi.uio.no

Phone +47 22856576

Username

Visiting address Sem Sælands vei 3 Farmasibygningen 0371 Oslo

Postal address Postboks 1068 Blindern 0316 Oslo

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Research

Our research is in the area of pharmaceutical analytical chemistry, and is focused on development of new microextraction concepts. Currently, our activities are related to electromembrane extraction (EME) and liquid-phase microextraction (LPME). EME and LPME can be used to isolate and pre-concentrate drug substances, drug metabolites, endogenous metabolites and peptides from human blood, urine, and saliva.

Our research is conceptual and balanced between fundamental and applied science, including the following activities:

Proof-of-principle experiments
Development of mass transfer theory
Development of equipment (including 96-well and microchip technology)
Development of forefront pharmaceutical and biomedical applications
Commercialization

Our research is motivated by the expectation that next generation analytical chemistry will largely rely on microextraction technologies for sample preparation.

Teaching

Teaching is within pharmaceutical analytical chemistry, and is focused on the following activities:

Chemical testing of pharmaceutical ingredients
Chemical testing of pharmaceutical preparations
Determination of drugs in biological fluids
General analytical chemistry

Author of international student textbooks (Wiley) in pharmaceutical analytical chemistry and bioanalysis

Higher education and employment history

Professor (part time) Department of Pharmacy, University of Copenhagen (Denmark) (2008-)
Professor, School of Pharmacy, University of Oslo (2000-)
Associate Professor, School of Pharmacy, University of Oslo (1996-2000)
PhD in Analytical Chemistry, University of Oslo (1995)
MSc in Chemistry, Technical University of Denmark (1990)

Appointments

Contributing Editor Trends in Analytical Chemistry
Associate Editor Journal of Pharmaceutical Analysis
Editor International Journal of Analytical Chemistry

Zeraatkar Moghaddam, Ali; Tabani, Hadi; Bameri, Amirehsan; Arabi, Elahe; Alexovič, Michal & Pedersen-Bjergaard, Stig (2024). Agarose-based gel electromembrane extraction using silica nanoparticles coated with polymeric deep eutectic solvent as a membrane additive. Journal of Molecular Liquids. ISSN 0167-7322. 401. doi: 10.1016/j.molliq.2024.124615.
Fabris, André Luis; Pedersen-Bjergaard, Stig; Øiestad, Elisabeth Leere; Rossi, Giordano Novak; Hallak, Jaime E.Cecílio & dos Santos, Rafael Guimarães [Show all 8 contributors for this article] (2024). Solvent-free parallel artificial liquid membrane extraction for drugs of abuse in plasma samples using LC-MS/MS. Analytica Chimica Acta. ISSN 0003-2670. 1301. doi: 10.1016/j.aca.2024.342387.
Fabris, André L.; Hansen, Frederik Andre; Yonamine, Mauricio; Pedersen-Bjergaard, Stig & Øiestad, Elisabeth Leere (2024). An efficient and green method for the analysis of synthetic cathinones in whole blood using 96-well electromembrane extraction and LC-MS/MS. Sustainable Chemistry and Pharmacy. ISSN 2352-5541. 38, p. 1–12. doi: 10.1016/j.scp.2024.101494.
Rye, Torstein Kige; Dowlatshah, Samira; Hansen, Frederik Andre; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2024). Electromembrane extraction of peptides – Looking retrospectively into published studies. Advances in Sample Preparation. ISSN 2772-5820. 9. doi: 10.1016/j.sampre.2024.100106.
Dowlatshah, Samira; Rye, Torstein Kige; Hansen, Frederik Andre; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2024). Parallel electromembrane extraction of peptides with monoterpene and medium-length fatty acid deep eutectic solvents. Analytica Chimica Acta. ISSN 0003-2670. 1297. doi: 10.1016/j.aca.2024.342360.
Song, Chenchen; Zhou, Chen; Hansen, Frederik Andre; Hay, Anne Oldeide & Pedersen-Bjergaard, Stig (2024). Optimization of generic conditions for electromembrane extraction of basic substances of moderate or low polarity. Journal of Separation Science. ISSN 1615-9306. 47(3). doi: 10.1002/jssc.202300801.
Zhou, Chen; Dowlatshah, Samira; Hansen, Frederik Andre & Pedersen-Bjergaard, Stig (2024). Generic conditions for electromembrane extraction of polar bases. Talanta: The International Journal of Pure and Applied Analytical Chemistry. ISSN 0039-9140. 267. doi: 10.1016/j.talanta.2023.125215.
Schüller, Maria; Lucic, Ivana; Øiestad, Åse Marit Leere; Pedersen-Bjergaard, Stig & Øiestad, Elisabeth Leere (2023). High-throughput quantification of emerging “nitazene” benzimidazole opioid analogs by microextraction and UHPLC–MS-MS . Journal of Analytical Toxicology. ISSN 0146-4760. 47(9), p. 787–796. doi: 10.1093/jat/bkad071. Full text in Research Archive
Schüller, Maria; Hansen, Frederik Andre & Pedersen-Bjergaard, Stig (2023). Extraction performance of electromembrane extraction and liquid-phase microextraction in prototype equipment. Journal of Chromatography A. ISSN 0021-9673. 1710. doi: 10.1016/j.chroma.2023.464440.
Martín, Alejandro; Dowlatshah, Samira; Pedersen-Bjergaard, Stig & Ramos-Payán, María (2023). Enhancing liquid phase microextraction enrichment in microchip devices under semi-stagnant conditions. Microchemical Journal. ISSN 0026-265X. 195. doi: 10.1016/j.microc.2023.109383. Full text in Research Archive
Dowlatshah, Samira; Hansen, Frederik Andre; Zhou, Chen; Ramos-Payán, María; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2023). Electromembrane extraction of peptides based on hydrogen bond interactions. Analytica Chimica Acta. ISSN 0003-2670. 1275. doi: 10.1016/j.aca.2023.341610. Full text in Research Archive
Zhou, Chen; Dowlatshah, Samira; Hay, Anne Oldeide; Schüller, Maria; Pedersen-Bjergaard, Stig & Hansen, Frederik Andre (2023). Generic Liquid Membranes for Electromembrane Extraction of Bases with Low or Moderate Hydrophilicity. Analytical Chemistry. ISSN 0003-2700. 95(23), p. 8982–8989. doi: 10.1021/acs.analchem.3c01052. Full text in Research Archive
Schüller, Maria; Hansen, Frederik Andre; Skaalvik, Tonje Gottenberg & Pedersen-Bjergaard, Stig (2023). Conductive vial electromembrane extraction – Principles and practical operation. Analytical Science Advances (ASA). ISSN 2628-5452. 4(7-8), p. 236–243. doi: 10.1002/ansa.202200065. Full text in Research Archive
Skaalvik, Tonje Gottenberg; Zhou, Chen; Øiestad, Elisabeth Leere; Hegstad, Solfrid; Trones, Roger & Pedersen-Bjergaard, Stig (2023). Conductive vial electromembrane extraction of opioids from oral fluid. Analytical and Bioanalytical Chemistry. ISSN 1618-2642. 415, p. 5323–5335. doi: 10.1007/s00216-023-04807-3. Full text in Research Archive
Tabani, Hadi; Dorabadizare, Farzaneh & Pedersen-Bjergaard, Stig (2023). Gel electro-membrane extraction: An overview on recent strategies for extraction efficiency enhancement. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 160. doi: 10.1016/j.trac.2023.116990.
Skaalvik, Tonje Gottenberg; Øiestad, Elisabeth Leere; Pedersen-Bjergaard, Stig & Hegstad, Solfrid (2023). Determination of amphetamine enantiomers in urine by conductive vial electromembrane extraction and ultra-high performance supercritical fluid chromatography tandem mass spectrometry. Drug Testing and Analysis. ISSN 1942-7603. 15, p. 909–918. doi: 10.1002/dta.3487. Full text in Research Archive
Schüller, Maria; McQuade, Tao Angell-Petersen; Bergh, Marianne Skov-Skov; Pedersen-Bjergaard, Stig & Øiestad, Elisabeth Leere (2023). Determination of tryptamine analogs in whole blood by 96-well electromembrane extraction and UHPLC-MS/MS. Talanta Open. ISSN 2666-8319. 7. doi: 10.1016/j.talo.2022.100171.
Hay, Anne Oldeide; Hansen, Frederik Andre; Psillakis, Elefteria & Pedersen-Bjergaard, Stig (2022). Liquid-phase microextraction in bioanalysis – How green can it be? Green Analytical Chemistry. ISSN 2772-5774. 3. doi: 10.1016/j.greeac.2022.100028.
Hansen, Frederik Andre & Pedersen-Bjergaard, Stig (2022). Electromembrane extraction – looking closer into the liquid membrane. Advances in Sample Preparation. ISSN 2772-5820. 2. doi: 10.1016/j.sampre.2022.100020.
Santigosa, Elia; Pedersen-Bjergaard, Stig; Giménez-Gómez, Pablo; Muñoz, María & Ramos-Payán, María (2022). A rapid and versatile microfluidic method for the simultaneous extraction of polar and non-polar basic pharmaceuticals from human urine. Analytica Chimica Acta. ISSN 0003-2670. 1208. doi: 10.1016/j.aca.2022.339829. Full text in Research Archive
López-Lorente, Ángela I.; Pena-Pereira, Francisco; Pedersen-Bjergaard, Stig; Zuin, Vânia G.; Ozkan, Sibel A. & Psillakis, Elefteria (2022). The ten principles of green sample preparation. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 148. doi: 10.1016/j.trac.2022.116530. Full text in Research Archive
Bavlovič Piskáčková, Hana; Nemeškalová, Alžběta; Kučera, Radim; Pedersen-Bjergaard, Stig; Najmanová, Věra & Štěrbová-Kovaříková, Petra [Show all 8 contributors for this article] (2022). Advanced microextraction techniques for the analysis of amphetamines in human breast milk and their comparison with conventional methods. Journal of Pharmaceutical and Biomedical Analysis. ISSN 0731-7085. 210. doi: 10.1016/j.jpba.2021.114549.
Hong, Changbao; Dong, Ying; Zhu, Ruiqin; Yan, Yibo; Shen, Xiantao & Pedersen-Bjergaard, Stig [Show all 7 contributors for this article] (2022). Effect of sample matrices on supported liquid membrane: Efficient electromembrane extraction of cathinones from biological samples. Talanta: The International Journal of Pure and Applied Analytical Chemistry. ISSN 0039-9140. 240. doi: 10.1016/j.talanta.2021.123175.
Hansen, Frederik Andre; Petersen, Nickolaj Jacob; Kutter, Jörg P. & Pedersen-Bjergaard, Stig (2022). Electromembrane extraction in microfluidic formats. Journal of Separation Science. ISSN 1615-9306. 45(1), p. 246–257. doi: 10.1002/jssc.202100603. Full text in Research Archive
Skottvoll, Frøydis Sved; Aizenshtadt, Aleksandra; Hansen, Frederik Andre; Amirola Martinez, Mikel; Andersen, Jannike Mørch & Bogen, Inger Lise [Show all 11 contributors for this article] (2022). Direct Electromembrane Extraction-Based Mass Spectrometry: A Tool for Studying Drug Metabolism Properties of Liver Organoids. Analysis & Sensing. ISSN 2629-2742. 2(2). doi: 10.1002/anse.202100051. Full text in Research Archive
Schüller, Maria; Tran, Kim Tu Thi; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2021). Membrane-based liquid-phase microextraction of basic pharmaceuticals – A study on the optimal extraction window. Journal of Chromatography A. ISSN 0021-9673. 1664(462769). doi: 10.1016/j.chroma.2021.462769. Full text in Research Archive
Mestad, Ingrid Oksavik; Gjelstad, Astrid; Pedersen-Bjergaard, Stig & Øiestad, Elisabeth Leere (2021). Green and sustainable drug analysis – Combining microsampling and microextraction of drugs of abuse. Sustainable Chemistry and Pharmacy. ISSN 2352-5541. 24. doi: 10.1016/j.scp.2021.100517. Full text in Research Archive
Lin, Bin; Liu, Huajing; Huang, Chuixiu; Xiao, Xianjin; Pedersen-Bjergaard, Stig & Shen, Xiantao (2021). Versatile Integration of Liquid-Phase Microextraction and Fluorescent Aptamer Beacons: A Synergistic Effect for Bioanalysis. Analytical Chemistry. ISSN 0003-2700. 93(42), p. 14323–14333. doi: 10.1021/acs.analchem.1c03600.
Eie, Linda Vårdal; Pedersen-Bjergaard, Stig & Hansen, Frederik Andre (2021). Electromembrane extraction of polar substances – Status and perspectives. Journal of Pharmaceutical and Biomedical Analysis. ISSN 0731-7085. 207. doi: 10.1016/j.jpba.2021.114407. Full text in Research Archive
Dowlatshah, Samira; Saraji, Mohammad; Pedersen-Bjergaard, Stig & Ramos Payán, Maria (2021). Microfluidic liquid-phase microextraction based on natural deep eutectic solvents immobilized in agarose membranes. Journal of Chromatography A. ISSN 0021-9673. 1657. doi: 10.1016/j.chroma.2021.462580.
Skaalvik, Tonje Gottenberg; Øiestad, Elisabeth Leere; Trones, Roger; Pedersen-Bjergaard, Stig & Hegstad, Solfrid (2021). Determination of psychoactive drugs in serum using conductive vial electromembrane extraction combined with UHPLC-MS/MS. Journal of chromatography. B. ISSN 1570-0232. 1183. doi: 10.1016/j.jchromb.2021.122926. Full text in Research Archive Show summary
Conductive vial electromembrane extraction (EME) with prototype equipment was applied for the first time to extract lipophilic basic drugs from serum. With this equipment, traditional platinum electrodes were replaced with sample and acceptor vials made from a conductive polymer, making the electrodes fully integrated and disposable. EME was combined with UHPLC-MS/MS, and a method to determine selected psychoactive drugs (alimemazine, amitriptyline, atomoxetine, clomipramine, doxepin, duloxetine, fluvoxamine, levomepromazine, nortriptyline and trimipramine) and metabolites (desmethyl clomipramine and desmethyl doxepin) in serum was developed, optimized, and validated. Extractions were carried out with 50 V for 15 min from serum samples (100 µL) diluted 1:3 with formic acid (0.1% v/v), using 2-nitrophenyl octyl ether as the supported liquid membrane (SLM), and formic acid (0.1% v/v, 300 µL) as acceptor phase. Using conductive vial EME, the extraction of lipophilic drugs reached exhaustive or near-exhaustive conditions, with recoveries in the range 75–117%. The method demonstrated excellent accuracy and precision, with bias within ± 6%, and intra- and inter-day CVs ranging 0.9 – 6% and 2 – 6%, respectively. In addition, acceptor phases were completely free of glycerophosphocholines. EME-UHPLC-MS/MS was successfully applied in determination of psychoactive drugs in 30 patient samples, and the results were in agreement with the current hospital routine method at St. Olav University Hospital (Trondheim, Norway). Obtaining comparable results to well-established routine methods is highly important for future implementation of EME into routine laboratories. These results thus serve as motivation for further advancing the EME technology. Until now, EME has been carried out with laboratory-build equipment, and the introduction of commercially available standardized equipment is expected to have a positive impact on future research activity.
Santigosa-Murillo, Elia; Pedersen-Bjergaard, Stig; Munoz, Maria & Ramos-Payan, Maria (2021). Green microfluidic liquid-phase microextraction of polar and non-polar acids from urine. Analytical and Bioanalytical Chemistry. ISSN 1618-2642. 413, p. 3717–3723. doi: 10.1007/s00216-021-03320-9.
Pedersen-Bjergaard, Stig (2021). Quality papers on sample preparation and extraction. Talanta: The International Journal of Pure and Applied Analytical Chemistry. ISSN 0039-9140. 3. doi: 10.1016/j.talo.2021.100043. Full text in Research Archive
Liu, Cong; Huang, Daixin; Yang, Liuqian; Wu, Shifan; Shen, Xiantao & Pedersen-Bjergaard, Stig [Show all 7 contributors for this article] (2021). Removal of Polymerase Chain Reaction Inhibitors by Electromembrane Extraction. Analytical Chemistry. ISSN 0003-2700. 93(33), p. 11488–11496. doi: 10.1021/acs.analchem.1c01689.
Rye, Torstein Kige; Martinovic, Gordana; Eie, Linda Vårdal; Hansen, Frederik Andre; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2021). Electromembrane extraction of peptides using deep eutectic solvents as liquid membrane. Analytica Chimica Acta. ISSN 0003-2670. 1175. doi: 10.1016/j.aca.2021.338717. Full text in Research Archive
Bavlovic Piskackova, Hana; Kollárová-Brázdová, Petra; Kučera, Radim; Macháček, Miloslav; Pedersen-Bjergaard, Stig & Štěrbová-Kovaříková, Petra (2021). The electromembrane extraction of pharmaceutical compounds from animal tissues. Analytica Chimica Acta. ISSN 0003-2670. 1177. doi: 10.1016/j.aca.2021.338742.
Hansen, Frederik Andre; Tirandaz, Shima & Pedersen-Bjergaard, Stig (2021). Selectivity and efficiency of electromembrane extraction of polar bases with different liquid membranes—Link to analyte properties. Journal of Separation Science. ISSN 1615-9306. 44(13), p. 2631–2641. doi: 10.1002/jssc.202100167.
Shang, Qianqian; Mei, Hang; Feng, Xinrui; Huang, Chuixiu; Pedersen-Bjergaard, Stig & Shen, Xiantao (2021). Ultrasound-assisted electromembrane extraction with supported semi-liquid membrane. Analytica Chimica Acta. ISSN 0003-2670. 1184. doi: 10.1016/j.aca.2021.339038.
Mei, Hang; Liu, Huajing; Shang, Qianqian; Dong, Ying; Pedersen-Bjergaard, Stig & Huang, Chuixiu [Show all 7 contributors for this article] (2021). Organic-solvent-free electromembrane extraction based on semi-interpenetrating polymer networks. Green Chemistry. ISSN 1463-9262. 23(4), p. 1782–1793. doi: 10.1039/d1gc00148e.
Skottvoll, Frøydis Sved; Hansen, Frederik; Harrison, Sean; Boger, Ida Caroline Sneis; Mrsa, Ago & Restan, Magnus Saed [Show all 14 contributors for this article] (2021). Electromembrane extraction and mass spectrometry for liver organoid drug metabolism studies. Analytical Chemistry. ISSN 0003-2700. 93, p. 3576–3585. doi: 10.1021/acs.analchem.0c05082. Full text in Research Archive
Eie, Linda Vårdal; Rye, Torstein Kige; Hansen, Frederik; Halvorsen, Trine Grønhaug & Pedersen-Bjergaard, Stig (2021). Electromembrane extraction of peptides and amino acids – status and perspectives. Bioanalysis. ISSN 1757-6180. 13(4), p. 277–289. doi: 10.4155/bio-2020-0285.
Hansen, Frederik & Pedersen-Bjergaard, Stig (2021). Electromembrane extraction of streptomycin from biological fluids. Journal of Chromatography A. ISSN 0021-9673. 1639. doi: 10.1016/j.chroma.2021.461915. Full text in Research Archive
Hansen, Frederik; Santigosa-Murillo, Elia; Payan, Maria Ramos; Munoz, Maria; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction using deep eutectic solvents as the liquid membrane. Analytica Chimica Acta. ISSN 0003-2670. 1143, p. 109–116. doi: 10.1016/j.aca.2020.11.044. Full text in Research Archive
Bavlovic Piskackova, Hana; Øiestad, Elisabeth Leere; Vanova, Nela; Lengvarska, Julia; Štěrbová-Kovaříková, Petra & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction of anthracyclines from plasma: Comparison with conventional extraction techniques. Talanta: The International Journal of Pure and Applied Analytical Chemistry. ISSN 0039-9140. 223. doi: 10.1016/j.talanta.2020.121748.
Drobnjak, Milena; Hansen, Frederik; Øiestad, Elisabeth Leere; Løvli, Trond; Trones, Roger & Martinsen, Ørjan Grøttem [Show all 7 contributors for this article] (2020). Electromembrane extraction with vials of conducting polymer. LC GC North America. ISSN 1527-5949. 38(8), p. 435–439.
Restan, Magnus Saed; Skottvoll, Frøydis Sved; Jensen, Henrik & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction of sodium dodecyl sulfate from highly concentrated solutions. The Analyst. ISSN 0003-2654. 145, p. 4957–4963. doi: 10.1039/d0an00622j.
Restan, Magnus Saed; Ramsrud, Sindre Bergstrøm; Jensen, Henrik & Pedersen-Bjergaard, Stig (2020). Influence of acid‐base dissociation equilibria during electromembrane extraction. Journal of Separation Science. ISSN 1615-9306. 43, p. 3120–3128. doi: 10.1002/jssc.202000391.
Hansen, Frederik; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2020). Bioanalysis of pharmaceuticals using liquid-phase microextraction combined with liquid chromatography–mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis. ISSN 0731-7085. 189, p. 1–12. doi: 10.1016/j.jpba.2020.113446.
Hansen, Frederik; Jensen, Henrik & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction using sacrificial electrodes. Analytical Chemistry. ISSN 0003-2700. 92(7), p. 5595–5603. doi: 10.1021/acs.analchem.0c00626.
Fashi, Armin; Cheraghi, Mahdi; Ebadipur, Hossein; Ebadipur, Hasan; Zamani, Abbasali & Badiee, Hamid [Show all 7 contributors for this article] (2020). Exploiting agarose gel modified with glucose-fructose syrup as a green sorbent in rotating-disk sorptive extraction technique for the determination of trace malondialdehyde in biological and food samples. Talanta: The International Journal of Pure and Applied Analytical Chemistry. ISSN 0039-9140. 217. doi: 10.1016/j.talanta.2020.121001.
Hansen, Frederik; Jaghl, Mohamad Fadi; Øiestad, Elisabeth Leere; Jensen, Henrik; Pedersen-Bjergaard, Stig & Huang, Chuixiu (2020). Impact of ion balance in electromembrane extraction. Analytica Chimica Acta. ISSN 0003-2670. 1124, p. 129–136. doi: 10.1016/j.aca.2020.05.039.
Hansen, Frederik; Kuban, Pavel; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2020). Electromembrane extraction of highly polar bases from biological samples – Deeper insight into bis(2-ethylhexyl) phosphate as ionic carrier. Analytica Chimica Acta. ISSN 0003-2670. 1115, p. 23–32. doi: 10.1016/j.aca.2020.04.027.
Abdel-Rehim, Mohamed; Pedersen-Bjergaard, Stig; Abdel-Rehim, Abbi; Lucena, Rafael; Moein, Mohammad Mahdi & Cardenas, Soledad [Show all 7 contributors for this article] (2020). Microextraction approaches for bioanalytical applications: An overview. Journal of Chromatography A. ISSN 0021-9673. 1616. doi: 10.1016/j.chroma.2019.460790.
Restan, Magnus Saed; Skjærvø, Øystein; Martinsen, Ørjan Grøttem & Pedersen-Bjergaard, Stig (2020). Towards exhaustive electromembrane extraction under stagnant conditions. Analytica Chimica Acta. ISSN 0003-2670. 1104, p. 1–9. doi: 10.1016/j.aca.2020.01.058. Full text in Research Archive
Dong, Ying; Li, Jing; Pedersen-Bjergaard, Stig & Huang, Chuixiu (2020). Unidirectional solute transfer using a Janus membrane. Journal of Membrane Science. ISSN 0376-7388. 596, p. 1–7. doi: 10.1016/j.memsci.2019.117723.
Khan, Wajid Ali; Arain, Muhammad Balal; Yamini, Yadollah; Shah, Nasrullah; Kazi, Tasneem Gul & Pedersen-Bjergaard, Stig [Show all 7 contributors for this article] (2020). Hollow fiber-based liquid phase microextraction followed by analytical instrumental techniques for quantitative analysis of heavy metal ions and pharmaceuticals. Journal of Pharmaceutical Analysis. ISSN 2095-1779. 10(2), p. 109–122. doi: 10.1016/j.jpha.2019.12.003.
Hansen, Frederik & Pedersen-Bjergaard, Stig (2019). Emerging extraction strategies in analytical chemistry. Analytical Chemistry. ISSN 0003-2700. 92(1), p. 2–15. doi: 10.1021/acs.analchem.9b04677.
Lin, Ann; Skottvoll, Frøydis Sved; Rayner, Simon; Pedersen-Bjergaard, Stig; Sullivan, Gareth & Krauss, Stefan [Show all 8 contributors for this article] (2019). 3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry. Electrophoresis. ISSN 0173-0835. 41, p. 56–64. doi: 10.1002/elps.201900170. Full text in Research Archive
Wan, Libin; Lin, Bin; Zhu, Ruiqin; Huang, Chuixiu; Pedersen-Bjergaard, Stig & Shen, Xiantao (2019). Liquid-Phase Microextraction or Electromembrane Extraction? Analytical Chemistry. ISSN 0003-2700. 91, p. 8267–8273. doi: 10.1021/acs.analchem.9b00946.
Pedersen-Bjergaard, Stig (2019). Analytical microextraction – Present status and future directions. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 118, p. 770–770. doi: 10.1016/j.trac.2019.07.004.
Rezazadeh, Maryam; Seidi, Shahram; Lid, Marthe; Pedersen-Bjergaard, Stig & Yamini, Yadollah (2019). The modern role of smartphones in analytical chemistry. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 118, p. 548–555. doi: 10.1016/j.trac.2019.06.019.
Ask, Kristine Skoglund; Lid, Marthe; Øiestad, Elisabeth Leere; Pedersen-Bjergaard, Stig & Gjelstad, Astrid (2019). Liquid-phase microextraction in 96-well plates - calibration and accurate quantification of pharmaceuticals in human plasma samples. Journal of Chromatography A. ISSN 0021-9673. 1602, p. 117–123. doi: 10.1016/j.chroma.2019.06.013.
Roman-Hidalgo, Cristina; Santigosa-Murillo, Elia; Ramos-Payan, Maria; Petersen, Nickolaj Jacob; Kutter, Jörg P. & Pedersen-Bjergaard, Stig (2019). On-chip electromembrane extraction of acidic drugs. Electrophoresis. ISSN 0173-0835. 40(18-19), p. 2514–2521. doi: 10.1002/elps.201900024.
Restan, Magnus Saed; Pedersen, Morten E.; Jensen, Henrik & Pedersen-Bjergaard, Stig (2019). Electromembrane Extraction of Unconjugated Fluorescein Isothiocyanate from Solutions of Labeled Proteins Prior to Flow Induced Dispersion Analysis. Analytical Chemistry. ISSN 0003-2700. 91(10), p. 6702–6708. doi: 10.1021/acs.analchem.9b00730.
Vårdal, Linda; Øiestad, Elisabeth Leere; Gjelstad, Astrid; Jensen, Henrik & Pedersen-Bjergaard, Stig (2019). Electromembrane extraction with solvent modification of the acceptor solution: improved mass transfer of drugs of abuse from human plasma. Bioanalysis. ISSN 1757-6180. 11(8), p. 755–771. doi: 10.4155/bio-2018-0308.
Pedersen-Bjergaard, Stig (2019). Electromembrane extraction – looking into the future. Analytical and Bioanalytical Chemistry. ISSN 1618-2642. 411(9), p. 1687–1693. doi: 10.1007/s00216-018-1512-x.
Drouin, Nicolas; Kuban, Pavel; Rudaz, Serge; Pedersen-Bjergaard, Stig & Schappler, Julie (2018). Electromembrane extraction: Overview of the last decade. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 113, p. 357–363. doi: 10.1016/j.trac.2018.10.024.
Hansen, Frederik A; Sticker, Drago; Kutter, Jorg P; Petersen, Nickolaj Jacob & Pedersen-Bjergaard, Stig (2018). Nanoliter-Scale Electromembrane Extraction and Enrichment in a Microfluidic Chip. Analytical Chemistry. ISSN 0003-2700. 90(15), p. 9322–9329. doi: 10.1021/acs.analchem.8b01936.
Lin, Bin; Wan, Libin; Sun, Xiaojie; Huang, Chuixiu; Pedersen-Bjergaard, Stig & Shen, Xiantao (2018). Electromembrane extraction of high level substances: A novel approach for selective recovery of templates in molecular imprinting. Journal of Membrane Science. ISSN 0376-7388. 568, p. 30–39. doi: 10.1016/j.memsci.2018.09.056.
Vårdal, Linda; Wong, Gladys; Øiestad, Åse Marit Leere; Pedersen-Bjergaard, Stig; Gjelstad, Astrid & Øiestad, Elisabeth Leere (2018). Rapid determination of designer benzodiazepines, benzodiazepines, and Z-hypnotics in whole blood using parallel artificial liquid membrane extraction and UHPLC-MS/MS. Analytical and Bioanalytical Chemistry. ISSN 1618-2642. 410(20), p. 4967–4978. doi: 10.1007/s00216-018-1147-y.
Vårdal, Linda; Øiestad, Elisabeth Leere; Gjelstad, Astrid & Pedersen-Bjergaard, Stig (2018). Electromembrane extraction of substances with weakly basic properties: A fundamental study with benzodiazepines. Bioanalysis. ISSN 1757-6180. 10(10), p. 769–781. doi: 10.4155/bio-2018-0030.
Ask, Kristine Skoglund; Øiestad, Elisabeth Leere; Pedersen-Bjergaard, Stig & Gjelstad, Astrid (2018). Dried blood spots and parallel artificial liquid membrane extraction – A simple combination of microsampling and microextraction. Analytica Chimica Acta. ISSN 0003-2670. 1009, p. 56–64. doi: 10.1016/j.aca.2018.01.024.
Olsen, Katharina Norgren; Ask, Kristine Skoglund; Pedersen-Bjergaard, Stig & Gjelstad, Astrid (2018). Parallel artificial liquid membrane extraction of psychoactive analytes: a novel approach in therapeutic drug monitoring. Bioanalysis. ISSN 1757-6180. 10(6), p. 385–395. doi: 10.4155/bio-2017-0250.
Dvorak, Milos; Seip, Knut Fredrik; Pedersen-Bjergaard, Stig & Kuban, Pavel (2018). Semi-automated set-up for exhaustive micro-electromembrane extractions of basic drugs from biological fluids. Analytica Chimica Acta. ISSN 0003-2670. 1005 , p. 34–42. doi: 10.1016/j.aca.2017.11.081.
Fuchs, David; Hidalgo, Cristina Román; Ramos Payán, Maria; Petersen, Nickolaj Jacob; Jensen, Henrik & Kutter, Jörg P. [Show all 7 contributors for this article] (2018). Continuous electromembrane extraction coupled with mass spectrometry – Perspectives and challenges. Analytica Chimica Acta. ISSN 0003-2670. 999, p. 27–36. doi: 10.1016/j.aca.2017.09.027.
Huang, Chuixiu; Shen, Xiantao; Gjelstad, Astrid & Pedersen-Bjergaard, Stig (2018). Investigation of alternative supported liquid membranes in electromembrane extraction of basic drugs from human plasma. Journal of Membrane Science. ISSN 0376-7388. 548, p. 176–183. doi: 10.1016/j.memsci.2017.11.001.
Huang, Chuixiu; Gjelstad, Astrid & Pedersen-Bjergaard, Stig (2017). Electromembrane extraction with alkylated phosphites and phosphates as supported liquid membranes. Journal of Membrane Science. ISSN 0376-7388. 526, p. 18–24. doi: 10.1016/j.memsci.2016.11.049.
Restan, Magnus Saed; Jensen, Henrik; Shen, Xiantao; Huang, Chuixiu; Martinsen, Ørjan Grøttem & Kubáň, Pavel [Show all 8 contributors for this article] (2017). Comprehensive study of buffer systems and local pH effects in electromembrane extraction. Analytica Chimica Acta. ISSN 0003-2670. 984, p. 116–123. doi: 10.1016/j.aca.2017.06.049.
Huang, Chuixiu; Chen, Zhiliang; Gjelstad, Astrid; Pedersen-Bjergaard, Stig & Shen, Xiantao (2017). Electromembrane extraction. TrAC. Trends in analytical chemistry. ISSN 0165-9936. 95, p. 47–56. doi: 10.1016/j.trac.2017.07.027.
Rye, Torstein Kige; Fuchs, David; Pedersen-Bjergaard, Stig & Petersen, Nickolaj Jacob (2017). Direct coupling of electromembrane extraction to mass spectrometry – Advancing the probe functionality toward measurements of zwitterionic drug metabolites . Analytica Chimica Acta. ISSN 0003-2670. 983, p. 121–129. doi: 10.1016/j.aca.2017.05.038.
Gjelstad, Astrid; Andresen, Alf Terje; Dahlgren, Anders; Gundersen, Thomas Erik & Pedersen-Bjergaard, Stig (2017). High-throughput liquid-liquid extraction in 96-well format: Parallel artificial liquid membrane extraction. LC GC North America. ISSN 1527-5949. 35(3), p. 192–196.
Fernández, Elena; Vårdal, Linda; Vidal, Lorena; Canals, Antonio; Gjelstad, Astrid & Pedersen-Bjergaard, Stig (2017). Complexation-mediated electromembrane extraction of highly polar basic drugs – a fundamental study with catecholamines in urine as model system . Analytical and Bioanalytical Chemistry. ISSN 1618-2642. 409, p. 4215–4223. doi: 10.1007/s00216-017-0370-2.
Pedersen-Bjergaard, Stig; Huang, Chuixiu & Gjelstad, Astrid (2017). Electromembrane extraction – Recent trends and where to go. Journal of Pharmaceutical Analysis. ISSN 2095-1779. 7(3), p. 141–147. doi: 10.1016/j.jpha.2017.04.002. Full text in Research Archive
Vårdal, Linda; Gjelstad, Astrid; Huang, Chuixiu; Øiestad, Elisabeth Leere & Pedersen-Bjergaard, Stig (2017). Efficient discrimination and removal of phospholipids during electromembrane extraction from human plasma samples. Bioanalysis. ISSN 1757-6180. 9(8), p. 631–641. doi: 10.4155/bio-2017-0027.

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Pedersen-Bjergaard, Stig; Gammelgaard, Bente & Halvorsen, Trine Grønhaug (2019). Introduction to Pharmaceutical Analytical Chemistry, 2nd Edition. Wiley-Blackwell. ISBN 9781119362722.

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Skottvoll, Frøydis Sved; Pedersen-Bjergaard, Stig; Mielnik, Michal Marek & Wilson, Steven Ray Haakon (2023). Electromembrane extraction merging with micro-nanotechnology for robust bridging of organ-on-a-chip and mass spectrometry.
Psillakis, Elefteria; Pedersen-Bjergaard, Stig & Ozkan, Sibel (2023). Editorial SI EuSP2022. Advances in Sample Preparation. ISSN 2772-5820. 5. doi: 10.1016/j.sampre.2023.100052.
Bull Tuner, Marius; Kogler, Stian; Hansen, Frederik Andre; Pedersen-Bjergaard, Stig; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2023). Exploring electromembrane extraction and LC-MS for studying liver organoid metabolomics.
Bull Tuner, Marius; Kogler, Stian; Hansen, Frederik Andre; Pedersen-Bjergaard, Stig; Røberg-Larsen, Hanne & Wilson, Steven Ray Haakon (2022). Exploring electromembrane extraction for sampling the organoid metabolome.
Psillakis, Elefteria; Pedersen-Bjergaard, Stig & Ozkan, Sibel A. (2022). Analytical Chemistry: There is no green like more green. LC GC Europe. ISSN 1471-6577. 35(10), p. 438–439. doi: 10.56530/lcgc.eu.fv1287o6.
Vik, Anders; Halvorsen, Trine Grønhaug; Primdahl, Karoline Gangestad; Hansen, Trond Vidar; Wangensteen, Helle & Reubsaet, Leon [Show all 7 contributors for this article] (2022). Grunnkurs i farmasøytisk kjemi på UiO moderniseres kontinuerlig. Norsk Farmaceutisk Tidsskrift. ISSN 0029-1935. p. 33–34.
Skottvoll, Frøydis Sved; Harrison, Sean; Hansen, Frederik; Pedersen-Bjergaard, Stig; Sullivan, Gareth & Bogen, Inger Lise [Show all 8 contributors for this article] (2020). Liver mini-organ analyses using liquid chromatography mass spectrometry. Show summary
Organoids (self-organized multi-cellular tissue derived from e.g. patient-derived stem cells) are emerging in vitro 3D representations of human organ functionality, predicted to have a game-changing impact on drug discovery, disease modeling and personalized medicine [1]. Integrating the organoids in a microfluidic chip (i.e. organoids-on-a-chip) would allow for controllable perfusion of nutrients and manipulation of the organoids by the addition of e.g. drugs [2]. Coupling the organoid-on-a-chip online to liquid chromatography mass spectrometry (LC-MS) would enable real-time measurements of the biochemical processes that occur on-chip. However, limited sample sizes, complex matrices and the presence of low abundant analytes also create analytical challenges [3]. Moreover, the organoids are still at the developmental stage, thus fundamental questions on the functionality of the developed organoids remain to be answered. Liver organoid functionality was assessed by protein characterization of adult liver tissue and liver organoids using nanoLC-MS/MS. The proteins identified from the liver organoids covered 70-84% (1003 proteins) of the liver protein expression from adult liver tissue derived from five patients. The proteins that were shared between the adult liver tissue and liver organoids could also relate to central liver pathways (e.g. amino acid biosynthesis and glycolysis). The sample preparation technique electromembrane extraction (i.e. electrophoretic separation across an oil membrane, EME) has shown to have on-chip analytical potential [4] and was thus used in the study of heroin drug metabolism in liver organoids. Multi-well EME (100 µL solutions) allowed for simple and repeatable monitoring of heroin metabolism kinetics without interference from cell medium components (e.g. albumin). The EME extracts were also compatible with different analytical approaches (LC-MS, nanoLC-MS, capillary electrophoresis-UV). Hence, the hyphenation of EME with nanoLC-MS would be a natural next step. References 1. Method of the Year 2017: Organoids. Nature Methods. Vol. 15 (2018) 1-1. 2. Park, S.E., A. Georgescu, and D. Huh, Organoids-on-a-chip. Science. Vol. 364 (2019) 960. 3. Lin, A., F. Sved Skottvoll, S. Rayner, S. Pedersen-Bjergaard, G. Sullivan, S. Krauss, S. Ray Wilson, and S. Harrison, 3D cell culture models and organ-on-a-chip: Meet separation science and mass spectrometry. ELECTROPHORESIS. Vol. 0 (2019). 4. Hansen, F.A., D. Sticker, J.P. Kutter, N.J. Petersen, and S. Pedersen-Bjergaard, Nanoliter-Scale Electromembrane Extraction and Enrichment in a Microfluidic Chip. Analytical Chemistry. Vol. 90 (2018) 9322-9329.
Pedersen-Bjergaard, Stig (2019). In-vitro drug metabolism monitoring by micro-chip electromembrane extraction coupled with mass spectrometry.
Skjærvø, Øystein; Pilarova, Veronika; Khalikova, Maria; Reubsaet, Leon; Halvorsen, Trine Grønhaug & Gjelstad, Astrid [Show all 10 contributors for this article] (2018). Those Who Can, Teach: Pharma Stars. The Analytical Scientist. ISSN 2051-4077. 1118, p. 40–45.
Pedersen-Bjergaard, Stig (2018). Microextraction based on electrical fields – combining partition and electrophoresis .
Pedersen-Bjergaard, Stig (2018). Liquid-phase microextraction – recent trends and perspectives .
Skjærvø, Øystein; Pilarova, Veronika; Khalikova, Maria; Reubsaet, Leon; Halvorsen, Trine Grønhaug & Gjelstad, Astrid [Show all 10 contributors for this article] (2018). Those Who Can, Teach: Pharma Stars. The Analytical Scientist. ISSN 2051-4077. 1118, p. 40–45.
Pedersen-Bjergaard, Stig & Gjelstad, Astrid (2017). Recent trends in drug analysis using liquid-phase microextraction.
Pedersen-Bjergaard, Stig & Gjelstad, Astrid (2017). Liquid-phase microextraction (LPME) – From conceptual development and into routine laboratories.
Pedersen-Bjergaard, Stig & Gjelstad, Astrid (2017). Microextraction through supported liquid membranes – Tuning the extraction chemistry for biomedical and pharmaceutical applications.

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Published Nov. 3, 2010 3:42 PM - Last modified Apr. 11, 2019 9:04 AM

Stig Pedersen-Bjergaard

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