2024
Revolutionizing biomarker analysis one tip at a time – coupling antibody to Volumetric Absorptive Microsamplers for sensitive LC-MS analysis
Johannsen C, Reubsaet L, Halvorsen TG
Adv. Sample Prep., 11 (2024) 100121, DOI: 10.1016/j.sampre.2024.100121
Advancements in Clinical Approaches, Analytical Methods and Smart Sampling for LC-MS based Protein Determination from Dried Matrix Spots
Reubsaet L, Halvorsen TG
J. Sep. Sci., 47 (2024) 2400061, DOI: 10.1002/jssc.202400061
Electromembrane extraction of peptides – looking retrospectively into published studies
Rye TK, Dowlatshah S, Hansen FA, Halvorsen TG, Pedersen-Bjergaard S
Adv. Sample Prep., 9 (2024) 100106, DOI: 10.1016/j.sampre.2024.100106
Parallel electromembrane extraction of peptides with monoterpene and medium-length fatty acid deep eutectic solvents
Dowlatshah S, Rye TK, Hansen FA, Halvorsen TG, Pedersen-Bjergaard S
Anal. Chim. Acta, 1297 (2024) 342360, DOI: 10.1016/j.aca.2024.342360
One-step functionalization of paper and simplified antibody immobilization for on-the-spot immunocapture from dried serum in LC-MS-based targeted protein determination
Mrsa A, Nardini G, Halvorsen TG, Thiede B, Reubsaet L
J. Mass Spectrom., 59 (2024) e4989, DOI: 10.1002/jms.4989
2023
Smart sampling as the 'spot-on' method for LC-MS protein analysis from dried blood spots
Johannsen C, Mrsa A, Halvorsen TG, Reubsaet L
J. Sep. Sci., 46 (2023) 2300394, DOI: 10.1002/jssc.202300394
The 25th Norwegian symposium on chromatography (editorial)
Malerød-Fjeld H, Halvorsen TG
Anal. Sci. Adv., 4 (2023) 223, DOI: 10.1002/ansa.202300035
Electromembrane extraction of peptides based on hydrogen bond interactions
Dowlatshah S, Hansen FA, Zhou C, Ramos-Payán M, Halvorsen TG, Pedersen-Bjergaard S
Anal. Chim. Acta, 1275 (2023) 341610, DOI: 10.1016/j.aca.2023.341610
Dried blood spot analysis with liquid chromatography and mass spectrometry: Trends in clinical chemistry
Skogvold HB, Rootwelt H, Reubsaet L, Elgstøen KBP, Wilson SR
J. Sep. Sci., (2023), DOI: 10.1002/jssc.202300210
Is it the end of dried blood spots as we know it?
Reubsaet L, Halvorsen TG
Anal. Sci. Adv., 4 (2023) 267, DOI: 10.1002/ansa.202300006
Microsampling in targeted mass spectrometry-based protein analysis of low-abundance proteins
Ngo HB, Oulie I, Reubsaet L, Halvorsen TG
J. Vis. Exp., 191 (2023) e64473, DOI: 10.3791/64473
2022
The utility of molecularly imprinted polymers for mass spectrometric protein and proteomics analysis
Halvorsen TG, Reubsaet L
Proteomics, 22 (2022) 2100395, DOI: 10.1002/pmic.202100395
Next generation VAMS® - trypsin immobilization for instant proteolysis in bottom-up protein determination
Reubsaet L, Thiede B, Halvorsen TG
Adv. Sample Prep., 3 (2022) 100027, DOI: 10.1016/j.sampre.2022.100027
On the spot immunocapture in targeted biomarker analysis using paper-bound streptavidin as anchor for biotinylated antibodies
Johannsen C, ul haq A, Reubsaet L, Halvorsen TG
Anal. Bioanal. Chem., 414 (2022) 5979, DOI: 10.1007/s00216-022-04161-w
Smart proteolysis samplers for pre-lab bottom-up protein analysis - performance of on-paper digestion compared to conventional digestion
Nguyen MT, Halvorsen TG, Thiede B, Reubsaet L
Sep. Sci. plus, 5 (2022) 171, DOI: 10.1002/sscp.202100062
2021
On-Line Duplex Molecularly Imprinted Solid-Phase Extraction for Analysis of Low-Abundant Biomarkers in Human Serum by Liquid Chromatography-Tandem Mass Spectrometry
McKitterick N, Bicak TC, Switnicka-Plak M, Cormack P, Reubsaet L, Halvorsen TG
J. Chromatogr. A, 1165 (2021) 462490, DOI: 10.1016/j.chroma.2021.462490
Affinity Capture in Bottom-up Protein Analysis – Overview of Current Status of Proteolytic Peptide Capture using Antibodies and Molecularly Imprinted Polymers
Halvorsen TG, McKitterick N, Kish M, Reubsaet L
Anal. Chim. Acta, 1182 (2021) 338714, DOI: 10.1016/j.aca.2021.338714
Electromembrane Extraction of Peptides using Deep Eutectic Solvents as Liquid Membrane
Rye TK, Martinovic G, Eie LV, Hansen FA, Halvorsen TG, Pedersen-Bjergaard S
Anal. Chim. Acta, 1175 (2021) 338717, DOI: 10.1016/j.aca.2021.338717
Next-Generation Dried Blood Spot Samplers for Protein Analysis: Describing Trypsin-Modified Smart Sampling Paper
Pizzi E, Halvorsen TG, Koehler CJ, Reubsaet L
Separations, 8 (2021) 66, DOI: 10.3390/separations8050066
Electromembrane extraction of peptides and amino acids – status and perspectives
Eie LV, Rye TK, Hansen F, Halvorsen TG, Pedersen-Bjergaard S
Bioanalysis, 13 (2021) 277, DOI: 10.4155/bio-2020-0285
Matrix-Assisted Ionization and Tandem Mass Spectrometry Capabilities in Protein Biomarker Characterization - An initial study using the Small Cell Lung Cancer Biomarker Progastrin Releasing Peptide as Model Compound
Halvorsen TG, Levernæs MCS, Rosting C
J. Am. Soc. Mass Spectrom., 32 (2021) 611, DOI: 10.1021/jasms.0c00336
Matrix assisted ionization mass spectrometry in targeted protein analysis – an initial evaluation
Skjærvø Ø, Trimpin S, Halvorsen TG
Rapid Commun. Mass Sepctrom., 35 (2021) e8437, DOI: 10.1002/rcm.8437
2020
Liquid chromatography mass spectrometry based characterization of epitope configurations
Levernæs MCS, Moe AU, Bøe S, Paus E, Reubsaet L, Halvorsen TG
Anal. Meth., 12 (2020) 5476, DOI: 10.1039/D0AY01283A
Facilitating Serum Determination of Neuron Specific Enolase at Clinically Relevant Levels by Coupling On-line Molecularly Imprinted Solid-Phase Extraction to LC-MS/MS
McKitterick N, Bicak TC, Cormack P, Reubsaet L, Halvorsen TG
Anal. Chim. Acta, 1140 (2020) 210, DOI: 10.1016/j.aca.2020.10.022
Magnetic Synthetic Receptors for Selective Clean-Up in Protein Biomarker Quantification
McKitterick N, Braathen F, Świtnicka-Plak MA, Reubsaet L, Cormack P, Halvorsen TG
J. Proteome Res., 19 (2020) 3573, DOI: 10.1021/acs.jproteome.0c00258
Dried Blood Spots in Mass Spectrometry-based Protein Analysis. Recent Developments including Sampling of other Biological Matrices and Novel Sampling Technologies
Halvorsen TG, Rosting C, Skjærvø Ø, Reubsaet L
LC GC Europe, 33 (2020) 222
Nano volume fractionation strategy for dilute-and-shoot injections in off-line loss-less proteomic workflows for extensive protein identifications of ultra-low sample amounts
Reubsaet L, Sweredoski M, Moradian A, Lomenick B, Eggleston-Rangel R, Garbis S
J. Chromatogr. A, 1609 (2020) 460507, DOI: 10.1016/j.chroma.2019.460507
Human Chorionic Gonadotropin Determination using Mass Spectrometry
Halvorsen TG, Reubsaet L
In Laurence A. Cole & Stephen A. Butler (ed.), 100 Years of Human Choronic Gonadotropin: Reviews and Perspectives. Elsevier. (2020) p. 123, DOI: 10.1016/B978-0-12-820050-6.00011-4
Pre-lab proteolysis for dried serum spots – a paper-based sampling concept targeting low abundant biomarkers
Skjærvø Ø, Halvorsen TG, Reubsaet L
Anal. Methods, 12 (2020) 97, DOI: 10.1039/C9AY01976F
2019
Immunocapture sample clean-up in determination of low abundant protein biomarkers – A feasibility study of peptide capture by anti-protein antibodies
Levernæs MCS, Farhat B, Oulie I, Abdullah SS, Paus E, Reubsaet L, Halvorsen TG
RCS Advances, 9 (2019) 34902, DOI: 10.1039/C9RA05071J
All-in-one paper-based sampling chip for targeted protein analysis
Skjærvø Ø, Halvorsen TG, Reubsaet L
Anal. Chim. Acta, 1089 (2019) 56, DOI: 10.1016/j.aca.2019.08.043
Data-Independent Acquisition for the Orbitrap Q Exactive HF: A Tutorial.
Reubsaet L, Sweredoski M, Moradian A
J. Proteome Res., 18 (2019) 803, DOI: 10.1021/acs.jproteome.8b00845
Paper-based immunocapture for targeted protein analysis
Skjærvø Ø, Solbakk EJ, Halvorsen TG, Reubsaet L
Talanta, 195 (2019) 764, DOI: 10.1016/j.talanta.2018.12.013
Introduction to pharmaceutical analytical chemistry
Pedersen-Bjergaard S, Gammelgaard B, Halvorsen TG
G.I.T. Laboratory Journal 3 (2019) 9
2018
Selective Fishing for Peptides with Antibody-Immobilized Acrylate Monoliths, Coupled Online with NanoLC-MS
Levernæs MCS, Brandtzæg OK, Amundsen SF, Reubsaet L, Lundanes E, Halvorsen TG, Wilson SR
Anal. Chem., 90 (2018) 13860, DOI: 10.1021/acs.analchem.8b00935
Smart blood spots for whole blood protein analysis
Skjærvø Ø, Halvorsen TG, Reubsaet L
Analyst, 143 (2018) 3184, DOI: 10.1039/C8AN00317C
Volumetric Absorptive MicroSampling vs. Other Blood Sampling Materials in LC-MS-based Protein Analysis – Preliminary Investigations
Andersen IKL, Rosting C, Gjelstad, Halvorsen TG
J. Pharm. Biomed. Anal., 156 (2018) 239, DOI: 10.1016/j.jpba.2018.036
Determination of the Low-Abundant Protein Biomarker hCG from Dried Matrix Spots using Immunocapture and Nano Liquid Chromatography Mass Spectrometry
Rosting C, Tran EV, Gjelstad, Halvorsen TG
J. Chromatogr. B, 1077-1078 (2018) 44, DOI: 10.1016/j.jchromb.2018.01.016
Those Who Can, Teach: Pharma Stars
Skjærvø Ø, Pilarova V, Khalikova M, Reubsaet L, Halvorsen TG, Gjelstad A, Øiestad EL , Lundanes E, Wilson SR, Pedersen-Bjergaard S
The Analytical Scientist, 70 (2018) 40
2017
Instant on-paper protein digestion during blood spot sampling
Skjærvø, Ø, Rosting C, Halvorsen TG, Reubsaet L
Analyst, 142 (2017) 3837, DOI: 10.1039/C7AN01075C
Antibody based affinity capture LC-MS/MS in quantitative determination of proteins in biological matrices
Halvorsen TG, Reubsaet L
Trends Anal. Chem., 95 (2017) 132, DOI: 10.1016/j.trac.2017.08.009
Exploring the peptide retention mechanism in molecularly imprinted polymers
Rossetti C, Ore OG, Sellergren B, Halvorsen TG, Reubsaet L
Anal. Bioanal. Chem., 409 (2017) 5631, DOI: 10.1007/s00216-017-0520-6
To elute or not to elute in immunocapture bottom-up LC–MS
Levernæs MCS, Broughton MN, Reubsaet L, Halvorsen TG
J. Chromatogr. B, 1055-1056 (2017) 51, DOI: 10.1016/j.jchromb.2017.03.044
Expanding the knowledge on Dried Blood Spots and LC-MS based protein analysis: Two different sampling materials and six protein targets
Rosting C, Gjelstad A, Halvorsen TG
Anal. Bioanal. Chem., 409 (2017) 3383, DOI: 10.1007/s00216-017-0280-3
Automated Protein Biomarker Analysis: on-line extraction of clinical samples by Molecularly Imprinted Polymers
Rossetti C, Świtnicka-Plak MA, Halvorsen TG, Cormack PAG, Sellergren B, Reubsaet L
Sci. Rep., 7 (2017) 44298, DOI: 10.1038/srep44298
Filter Plate–Based Screening of MIP SPE Materials for Capture of the Biomarker Pro-Gastrin-Releasing Peptide
Jagadeesan KK, Rossetti C, Qader AA, Reubsaet L, Sellergren B, Laurell L, Ekström S
SLAS Discov., 22 (2017) 1253, DOI: 10.1177/2472555216689494
2016
Isolation and mass spectrometry analysis of urinary extraexosomal proteins
Hildonen S, Skarpen E, Halvorsen TG, Reubsaet L
Sci. Rep., 6 (2016) 36331, DOI: 10.1038/srep3633
Evaluation of affinity-based serum clean-up in mass spectrometric analysis: plastic vs. monoclonal antibodies
Rossetti C, Levernæs MCS, Reubsaet L, Halvorsen TG
J. Chromatogr. A, 1471 (2016) 19, DOI: 10.1016/j.chroma.2016.09.069
Dual-immuno-MS technique for improved differentiation power in heterodimeric protein biomarker analysis: determination and differentiation of human chorionic gonadotropin variants in serum
Egeland SV, Paus E, Reubsaet L, Halvorsen TG
Anal. Bioanal. Chem., 408 (2016) 7379, DOI: 10.1007/s00216-016-9818-z
Evaluation of Water-Soluble Dried Blood Spots for Small Proteins: A Conceptual Study using Insulin as Model Analyte
Rosting C, Sæ CØ, Gjelstad A, Halvorsen TG
Bioanalysis, 8 (2016) 1051, DOI: 10.4155/bio-2016-0002
The pros and cons of increased trypsin-to-protein ratio in targeted protein analysis
Egeland SV, Reubsaet L, Halvorsen TG
J. Pharm. Biomed. Anal., 123 (2016) 155, DOI: 10.1016/j.jpba.2016.02.0115
Cyclic Diguanylate Regulation of Bacillus cereus Group Biofilm Formation
Fagerlund A, Smith V, Røhr ÅK, Lindbäk T, Parmer MP, Andersson KK, Reubsaet L, Økstad OA
Mol. Microbiol., 101 (2016) 471, DOI: 10.1111/mmi.13405
The impact of high dose acetylcholine on bovine corneal epithelium
Ringvold A, Reubsaet L
Acta Ophthalmol., 94 (2016) 160, DOI: 10.1111/aos.12889
Determination of Very Low-Abundance Diagnostic Proteins in Serum Using Immuno-Capture LC-MS-MS
Reubsaet L, Halvorsen TG
LC GC Europe, 29 (2016) 352
LC GC Asia Pacific, 19 (2016) 6
Curr. Trends Mass Spectrom., 15 (2017) 16
2015
Involvement of Covalent Interactions in the Mode of Action of PPARβ/δ Antagonists
Kaupang Å, Hildonen S, Halvorsen TG, Mortén M, Vik A, Hansen TV
RSC Advances, 5 (2015) 76483, DOI: 10.1039/C5RA15707B
Water-Soluble Dried Blood Spot in Protein Analysis: A Proof-of-Concept Study
Rosting C, Gjelstad A, Halvorsen TG
Anal. Chem., 87 (2015) 7918, DOI: 10.1021/acs.analchem.5b01735
Synthesis, biological evaluation and molecular modeling studies of the PPARβ/δ antagonist CC618
Kaupang Å, Paulsen SM, Steindal CC, Ravna AW, Sylte I, Halvorsen TG, Thoresen H, Hansen TV
Eur. J. Med. Chem., 94 (2015) 229, DOI: 10.1016/j.ejmech.2015.03.006
Vaccination with outer membrane vesicles from Francisella noatunensis reduces development of francisellosis in a zebrafish model
Brudal E, Lampe EO, Reubsaet L, Roos N, Hegna IK, Thrane IM, Koppang EO, Winther-Larsen HC
Fish Shellfish Immunol., 42 (2015) 50, DOI: 10.1016/j.fsi.2014.10.025
2014
Antibody-Free Biomarker Determination: Exploring Molecularly Imprinted Polymers for Pro Gastrin Releasing Peptide.
Rossetti C, Qader AA, Halvorsen TG, Sellergren B, Reubsaet L
Anal. Chem., 86 (2014) 12291, DOI: 10.1021/ac503559c
Why less is more when generating tryptic peptides in bottom-up proteomics
Hildonen S, Halvorsen TG, Reubsaet L
Proteomics, 14 (2014) 2031, DOI: 10.1002/pmic.201300479
A pilot study showing differences in glycosylation patterns of IgG subclasses induced by pneumococcal, meningococcal and two types of influenza vaccines
Vestrheim AC, Moen A, Egge-Jacobsen W, Reubsaet L, Halvorsen TG, Bratlie DB, Paulsen BS, Michaelsen TE
Immunity Inflam. Dis., 2 (2014) 76, DOI: 10.1002/iid3.22
Multiplexing determination of Small Cell Lung Cancer Biomarkers and their isovariants in serum by immunocapture LC-MS/MS
Torsetnes S, Levernæs M, Broughton M, Paus E, Halvorsen TG, Reubsaet L
Anal. Chem., 86 (2014) 6983, DOI: 10.1021/ac500986t
Determining ProGRP and isoforms in lung- and thyroid cancer patient samples. Comparing an MS method with a routine clinical immunoassay
Torsetnes SB, Nordlund MS, Paus E, Halvorsen TG, Reubsaet L
Anal. Bioanal. Chem., 406 (2014) 2733, DOI: 10.1007/s00216-014-7634-x
Epitope analysis and detection of human chorionic gonadotropin (hCG) variants by monoclonal antibodies and mass spectrometry
Lund H, Paus E, Berger P, Stenman U-H, Torcellini T, Halvorsen TG, Reubsaet L
Tumor Biol., 35 (2014) 1013, DOI: 10.1007/s13277-013-1135-y
Bradykinin analysis revived - A validated method for determination of its stable metabolite in whole blood by LC-MS/MS
Seip KF, Bjerknes KC, Johansen HT, Nielsen EW, Landrø LAY, Reubsaet L
J. Chromatogr. B, 947-948 (2014) 139, DOI: 10.1016/j.jchromb.2013.12.033
Peptide imprinted receptors for the determination of the small cell lung cancer associated biomarker progastrin releasing peptide
Qader AA, Urraca J, Torsetnes SB, Tønnesen F, Reubsaet L, Sellergren B
J. Chromatogr. A, 1370 (2014) 56, DOI: 10.1016/j.chroma.2014.10.023
2013
Immunocapture and LC-MS/MS for selective quantification and differentiation of the isozymes of the biomarker neuron-specific enolase in serum
Torsetnes SB, Løvbak SG, Claus C, Lund H, Nordlund MS, Paus E, Halvorsen TG, Reubsaet L
J. Chromatogr. B, 929 (2013) 125, DOI: 10.1016/j.jchromb.2013.04.010
Comparison of newly developed immuno-MS method with existing DELFIA® immunoassay for human chorionic gonadotropin determination in doping analysis
Lund H, Snilsberg A-H, Halvorsen TG, Hemmersbach P, Reubsaet L
Bioanalysis, 5 (2013) 623, DOI: 10.4155/bio.13.8
Sports Drug Testing using Immuno-MS: Clinical Study Comprising Administration of Human Chorionic Gonadotropin to Males
Lund H, Snilsberg A-H, Paus E, Halvorsen TG, Hemmersbach P, Reubsaet L
Anal. Bioanal. Chem., 405 (2013) 1569, DOI: 10.1007/s00216-012-6566-6
Digging deeper into the field of the SCLC-tumour marker ProGRP. A method for differentiation of its isoforms
Torsetnes S, Nordlund M, Paus E, Halvorsen TG, Reubsaet L
J. Proteome Res., 12 (2013) 412, DOI: 10.1021/pr300751j
Integrated enzyme reactor and high resolving chromatography in ‘‘sub-chip’’ dimensions for sensitive protein mass spectrometry
Hustoft HK, Brandtzæg OKM, Røgeberg M, Misaghian D, Torsetnes SB, Greibrokk T, Reubsaet L, Wilson SRH, Lundanes E
Sci. Rep., 3 (2013) 3511, DOI: 10.1038/srep03511
2012
Immuno-MS Based Targeted Proteomics: Highly Specific, Sensitive, and Reproducible Human Chorionic Gonadotropin Determination for Clinical Diagnostics and Doping Analysis
Lund H, Løvsletten K, Paus E, Halvorsen TG, Reubsaet L
Anal. Chem., 84 (2012) 7926, DOI: 10.1021/ac301418f
New labdane diterpenes from Solidago canadensis
Wangensteen H, Phan TT, Rise F, Halvorsen TG, Malterud KE
Nat. Prod. Res., 26 (2012) 1348, DOI: 10.1080/14786419.2011.565005
Fast, selective, and sensitive analysis of low-abundance peptides in human plasma by electromembrane extraction
Balchen M, Lund H, Reubsaet L, Pedersen-Bjergaard S
Anal. Chim. Acta, 716 (2012) 16-23, DOI: 10.1016/j.aca.2011.02.058
Urinary proteomic shotgun approach for identification of potential acute rejection biomarkers in renal transplant recipients
Loftheim H, Midtvedt K, Hartmann A, Reisæter AV, Falck Pål, Holdaas H, Jenssen TG, Reubsaet L, Åsberg A
Transplant. Res., 1 (2012) 9, DOI: 10.1186/2047-1440-1-9
A critical review of trypsin digestion for LC-MS based proteomics
Hustoft HK, Malerød H, Wilson SR, Reubsaet L, Lundanes E, Greibrokk T
In Eastwood Leung (ed.), Integrative Proteomics. InTech Open. (2012) p. 73, DOI: 10.5772/29326