Contact information:
Facility Manager: Nina Frederike J Edin
Location: Sem Sælands vei 26
0371 OSLO
Norway
Instruments:
- Cell laboratory with equipment for cell culture (class 2): bio safety cabinets, incubators, shaking incubator for suspension cells etc.
- 220 kV X-ray facility with dedicated cell irradiation set-up
- Cell irradiation set-up and bio safety cabinet with incubator in connection with Oslo Cyclotron Laboratory for proton irradiation
- Advanced dosimetry equipment (ionization chambers, gafchromic film, EPR)
- Hypoxia laboratory with 3 hypoxia chambers and an advanced sensor system for oxygen measurements
- Flow cytometer
- Plate reader
- Liquid scintillation counter
- Confocal microscope
Description of services:
- X-ray and proton irradiation of cell cultures with dosimetry measurements
- Studies of radiation responses
- Studies of effects of hypoxia including measurements of oxygen and metabolic compounds
About the Cell-Laboratory
We study the effect of ionizing radiation on cells, alone or in combination with hypoxia, chemotherapy or immune therapy. We have a focus on differential responses to proton versus X-ray irradiation. In addition, we have a particular interest in the effects of low doses and low dose rates. Various growth parameters (protein metabolism, number of cells, and cell cycle distribution) are studied and we use a number of different molecular biological techniques (western blotting, transfection, flow cytometry, immunocytochemistry).