Fundamental theory of radiobiology:
cell cycle, DNA damage by x-rays (direct/indirect action), DNA repair processes (double strand break repair), target theory and survival curves, spheroid formation (cell adhesion), hypoxia.
Experimental methods:
(HT29 cell line: colorectal denocarcinoma). Monolayer and spheroid cultures, irradiation, trypsinizing, single cell seeding and colony counting.
Results and conclusions:
Comparison of survival curves between monolayers and whole spheroids, comparison of survival curves between 4 different layers of spheroids (surface, layer 2nd , layer 3rd , core), comparison between survival curves from the same layers just after irradiation and 18 hours after irradiation. Presentation of histograms/hitogram data of the layers at 0 and 18 hours after irradiation.
Conclusions.