Ingunn Hanson

• Hanson, Ingunn; Vatne, Jenny Tovseth & Edin, Nina Frederike Jeppesen (2024). Effects of bone marrow sparing and TGF-β3 treatment in total body irradiation of C57BL/6J mice. Applied Biosciences. ISSN 2813-0464. doi: 10.3390/applbiosci3020011.
• Hanson, Ingunn; Juvkam, Inga; Zlygosteva, Olga; Søland, Tine Merete; Galtung, Hilde & Malinen, Eirik [Vis alle 7 forfattere av denne artikkelen] (2024). TGF-β3 increases the severity of radiation-induced oral mucositis and salivary gland fibrosis in a mouse model. International Journal of Radiation Biology. ISSN 0955-3002. doi: 10.1080/09553002.2024.2324476.
• Hanson, Ingunn; Pitman, Kathinka Elinor & Edin, Nina Frederike Jeppesen (2023). The Role of TGF-β3 in Radiation Response. International Journal of Molecular Sciences. ISSN 1661-6596. 24(8). doi: 10.3390/ijms24087614. Fulltekst i vitenarkiv
• Hanson, Ingunn; Pitman, Kathinka Elinor; Altanerova, Ursula; Altaner, Čestmír; Malinen, Eirik & Edin, Nina Frederike Jeppesen (2022). Low-Dose-Rate Radiation-Induced Secretion of TGF-β3 Together with an Activator in Small Extracellular Vesicles Modifies Low-Dose Hyper-Radiosensitivity through ALK1 Binding. International Journal of Molecular Sciences. ISSN 1661-6596. 23(15). doi: 10.3390/ijms23158147.
• Arous, Delmon; Schrunner, Stefan; Hanson, Ingunn; Edin, Nina Frederike Jeppesen & Malinen, Eirik (2022). Principal component-based image segmentation: a new approach to outline in vitro cell colonies. Computer Methods in Biomechanics and Biomedical Engineering: Imaging and Visualization. ISSN 2168-1163. doi: 10.1080/21681163.2022.2035822. Fulltekst i vitenarkiv
• Sulheim, Einar; Hanson, Ingunn; Snipstad, Sofie; Vikedal, Krister; Mørch, Ýrr Asbjørg & Boucher, Yves [Vis alle 7 forfattere av denne artikkelen] (2021). Sonopermeation with nanoparticle-stabilized microbubbles reduces solid stress and improves nanomedicine delivery to tumors. Advanced therapeutics. ISSN 2366-3987. 4(10). doi: 10.1002/adtp.202100147. Fulltekst i vitenarkiv Vis sammendrag

  Drug delivery to tumors is challenging due to biological barriers obstructing effective delivery. Sonopermeation with ultrasound and microbubbles has been shown to improve therapeutic effect of many classes of drugs, but the underlying mechanism is not fully understood. In this study, two subcutaneous xenograft tumor models, that differed substantially in blood vessel density and stiffness, is treated with poly(alkyl cyanoacrylate) nanoparticles and nanoparticle-stabilized microbubbles combined with ultrasound. Improved nanoparticle accumulation and extracellular matrix (ECM) penetration is found. The stiffness and solid stress in the tumors are measured and it is discovered that sonopermeation can reduce the solid stress in both models, with the highest effect in the stiffest tumor model. This suggests that sonopermeation affects not only the blood vessel wall which has been described previously, but also the ECM to reduce solid stress and increase diffusion and transport of nanomedicines.

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• Hanson, Ingunn; Juvkam, Inga; Zlygosteva, Olga & Edin, Nina Frederike Jeppesen (2023). TGF-β3 increases the severity of radiation-induced oral mucositis and salivary gland fibrosis in a mouse model.
• Hanson, Ingunn; Juvkam, Inga; Zlygosteva, Olga & Edin, Nina Frederike Jeppesen (2023). TGF-β3 increases the severity of radiation-induced oral mucositis and salivary gland fibrosis in a mouse model.
• Hanson, Ingunn & Edin, Nina Frederike Jeppesen (2022). Shielding of the Femur Reduces the Severity of the Gastrointestinal Subsyndrome of ARS and Increases Survival in Mice.
• Hanson, Ingunn & Edin, Nina Frederike Jeppesen (2022). The role of TGF-β3 in modification of radiation response.
• Hanson, Ingunn & Edin, Nina Frederike Jeppesen (2022). Shielding of the femur reduces the severity of the gastrointestinal subsyndrome of ARS and increases survival in mice.
• Hanson, Ingunn & Edin, Nina Frederike Jeppesen (2021). The role of TGF-B3 in modification of radiation response.
• Hanson, Ingunn; Pitman, Kathinka Elinor; Thiede, Bernd; Cai, Yiqing & Edin, Nina Frederike Jeppesen (2021). Proteomic Analysis of Low Dose-Rate Primed Cells and Extracellular Exosomes.
• Hanson, Ingunn; Edin, Nina Frederike J; Thiede, Bernd & Altanerova, Ursula (2020). Communication of radioprotective efects by TGF-β3.
• Hanson, Ingunn; Altanerova, Ursula; Thiede, Bernd & Edin, Nina Frederike J (2020). Radioprotector TGF-β3 is secreted in exosomes and activated by LDR priming. Vis sammendrag

  The biological effects of ionizing radiation in the low-dose region is important for diagnostic imaging, healthy tissue in radiation therapy and environmental radiation. For a majority of tested cell lines, the low dose hyper-radiosensitive (HRS) response has been shown to be default. We have previously shown that cells primed with a low dose-rate (⁓0.3 Gy/h) ionizing radiation permanently loses the HRS response. We have also shown that the transforming growth factor (TGF)-β3 is responsible for this effect. Here, we investigated the secretion mechanism of TGF-β3 from LDR primed cells, and theorize regarding the way it is activated. Exosomes were isolated from LDR primed cells, and were shown to remove HRS in reporter cells when co-cultured with these. Adding a TGF-β3 inhibitor to the medium restored the HRS response in reporter cells, confirming that TGF-β3 was the factor in the exosomes responsible for removing HRS. Mass spectrometry was performed on the content of exosomes from primed and control cells. Proteomic analysis confirmed the presence of exosome-specific proteins in exosomes from primed and control groups. TGF-β3 along with latency-associated protein (LAP), the protein that TGF-β3 is bound to in its inactive form, was present in similar amounts in both groups, indicating that TGF-β3 was secreted in its inactive form, regardless of LDR priming. Three proteins were significantly upregulated in the primed exosomes. These proteins will be investigated further in future experiments. We conclude that TGF-β3 is secreted in its inactive form in exosomes from LDR primed and unprimed cells, and that the priming is responsible for its activation. We will further investigate three significantly upregulated proteins with regards to their role in the activation of TGF-β3.

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