Luis N. Morgado

• Ransedokken, Yngvild Eidissen; Asplund, Johan; Morgado, Luis; Kauserud, Håvard; Mundra, Sunil & Ohlson, Mikael [Show all 8 contributors for this article] (2024). Synchronic shifts in phenolic compounds and fungal communities during litter decomposition in boreal forests. Forest Ecology and Management. ISSN 0378-1127. 554. doi: 10.1016/j.foreco.2024.121696.
• Fiore-Donno, Anna Maria; Human, Zander R.; Štursová, Martina; Mundra, Sunil; Morgado, Luis & Kauserud, Håvard [Show all 8 contributors for this article] (2022). Soil compartments (bulk soil, litter, root and rhizosphere) as main drivers of soil protistan communities distribution in forests with different nitrogen deposition. Soil Biology and Biochemistry. ISSN 0038-0717. 168. doi: 10.1016/j.soilbio.2022.108628. Full text in Research Archive
• Estensmo, Eva Lena Fjeld; Botnen, Synnøve Smebye; Maurice, Sundy; Martin Sanchez, Pedro Maria; Morgado, Luis & Engh, Ingeborg Bjorvand [Show all 9 contributors for this article] (2022). The Indoor Mycobiomes of Daycare Centers Are Affected by Occupancy and Climate. Applied and Environmental Microbiology. ISSN 0099-2240. 88(6). doi: 10.1128/aem.02113-21. Full text in Research Archive
• Estensmo, Eva Lena F.; Morgado, Luis; Maurice, Sundy; Martin Sanchez, Pedro Maria; Engh, Ingeborg B. & Mattsson, Johan [Show all 8 contributors for this article] (2021). Spatiotemporal variation of the indoor mycobiome in daycare centers. Microbiome. ISSN 2049-2618. 9(1), p. 1–12. doi: 10.1186/s40168-021-01167-x. Full text in Research Archive
• Mohamed, Nazar; Litlekalsøy, Jorunn; Ahmed, Israa Abdul Rhman ; Martinsen, Einar Marius Hjellestad; Furriol, Jessica & Javier-Lopez, Ruben [Show all 15 contributors for this article] (2021). Analysis of salivary mycobiome in a cohort of oral squamous cell carcinoma patients from Sudan identifies higher salivary carriage of malassezia as an independent and favorable predictor of overall survival. Frontiers in Cellular and Infection Microbiology. ISSN 2235-2988. 11, p. 1–15. doi: 10.3389/fcimb.2021.673465. Full text in Research Archive
• Geml, József; Morgado, Luis & Semenova-Nelsen, Tatiana A. (2021). Tundra Type Drives Distinct Trajectories of Functional and Taxonomic Composition of Arctic Fungal Communities in Response to Climate Change – Results From Long-Term Experimental Summer Warming and Increased Snow Depth. Frontiers in Microbiology. ISSN 1664-302X. 12, p. 1–13. doi: 10.3389/fmicb.2021.628746. Full text in Research Archive
• Tonjer, Lea-Rebekka; Thoen, Ella; Morgado N, Luis; Botnen, Synnøve; Mundra, Sunil & Nybakken, Line [Show all 8 contributors for this article] (2021). Fungal community dynamics across a forest–alpine ecotone. Molecular Ecology. ISSN 0962-1083. 30(19), p. 4926–4938. doi: 10.1111/mec.16095. Full text in Research Archive
• Danielsen, Jørgen Skyrud; Morgado, Luis; Mundra, Sunil; Nybakken, Line; Davey, Marie Louise & Kauserud, Håvard (2021). Establishment of spruce plantations in native birch forests reduces soil fungal diversity. FEMS Microbiology Ecology. ISSN 0168-6496. 97(7). doi: 10.1093/femsec/fiab074. Full text in Research Archive Show summary

  Plantations of Norway spruce have been established well beyond its natural range in many parts of the world, potentially impacting native microbial ecosystems and the processes they mediate. In this study, we investigate how the establishment of spruce plantations in a landscape dominated by native birch forests in western Norway impacts soil properties and belowground fungal communities. Soil cores were collected from neighboring stands of planted spruce and native birch forests. We used DNA metabarcoding of the rDNA internal transcribed spacer 2 region and ergosterol measurements to survey the fungal community composition and its biomass, respectively. In the two investigated soil layers (litter and humus), fungal community composition, diversity and biomass were strongly affected by the tree species shift. Native birch stands hosted markedly richer fungal communities, including numerous fungi not present in planted spruce stands. In contrast, the spruce stands included higher relative abundance of ectomycorrhizal fungi as well as higher fungal biomass. Hence, establishing plantations of Norway spruce in native birch forests leads to significant losses in diversity, but increase in biomass of ectomycorrhizal fungi, which could potentially impact carbon sequestration processes and ecosystem functioning. forest plantations, Norway spruce, soil fungi, ectomycorrhiza, fungal communities, DNA metabarcoding

• Mundra, Sunil; Kjønaas, O. Janne; Morgado, Luis; Krabberød, Anders Kristian; Ransedokken, Yngvild & Kauserud, Håvard (2021). Soil depth matters: shift in composition and inter-kingdom co-occurrence patterns of microorganisms in forest soils. FEMS Microbiology Ecology. ISSN 0168-6496. 97(3). doi: 10.1093/femsec/fiab022. Full text in Research Archive Show summary

  Soil depth represents a strong physiochemical gradient that greatly affects soil-dwelling microorganisms. Fungal communities are typically structured by soil depth, but how other microorganisms are structured is less known. Here, we tested whether depth-dependent variation in soil chemistry affects the distribution and co-occurrence patterns of soil microbial communities. This was investigated by DNA metabarcoding in conjunction with network analyses of bacteria, fungi, as well as other micro-eukaryotes, sampled in four different soil depths in Norwegian birch forests. Strong compositional turnover in microbial assemblages with soil depth was detected for all organismal groups. Significantly greater microbial diversity and fungal biomass appeared in the nutrient-rich organic layer, with sharp decrease towards the less nutrient-rich mineral zones. The proportions of copiotrophic bacteria, Arthropoda and Apicomplexa were markedly higher in the organic layer, while patterns were opposite for oligotrophic bacteria, Cercozoa, Ascomycota and ectomycorrhizal fungi. Network analyses indicated more intensive inter-kingdom co-occurrence patterns in the upper mineral layer (0–5 cm) compared to the above organic and the lower mineral soil, signifying substantial influence of soil depth on biotic interactions. This study supports the view that different microbial groups are adapted to different forest soil strata, with varying level of interactions along the depth gradient.

• Estensmo, Eva Lena Fjeld; Maurice, Sundy Ursula Mary Jane; Morgado, Luis; Martin-Sanchez, Pedro Maria; Skrede, Inger & Kauserud, Håvard (2021). The influence of intraspecific sequence variation during DNA metabarcoding: A case study of eleven fungal species. Molecular Ecology Resources. ISSN 1755-098X. doi: 10.1111/1755-0998.13329. Full text in Research Archive Show summary

  DNA metabarcoding has become a powerful approach for analysing complex communities from environmental samples, but there are still methodological challenges limiting its full potential. While conserved DNA markers, like 16S and 18S, often are not able to discriminate among closely related species, other more variable markers – like the fungal ITS region, may include considerable intraspecific variation, which can lead to oversplitting of species during DNA metabarcoding analyses. Here we assessed the effects of intraspecific sequence variation in DNA metabarcoding by analysing local populations of eleven fungal species. We investigated the allelic diversity of ITS2 haplotypes using both Sanger sequencing and high throughput sequencing (HTS) coupled with error correction with the software dada2. All the eleven species, except one, included some level of intraspecific variation in the ITS2 region. Overall, we observed a high correspondence between haplotypes generated by Sanger sequencing and HTS, with the exception of a few additional haplotypes detected using either approach. These extra haplotypes, typically occurring in low frequencies, were probably due to PCR and sequencing errors or intragenomic variation in the rDNA region. The presence of intraspecific (and possibly intragenomic) variation in ITS2 suggest that haplotypes (or ASVs) should not be used as basic units in ITS‐based fungal community analyses, but an extra clustering step is needed to approach species‐level resolution.

• Martin-Sanchez, Pedro Maria; Estensmo, Eva Lena Fjeld; Morgado, Luis; Maurice, Sundy Ursula Mary Jane; Engh, Ingeborg Bjorvand & Skrede, Inger [Show all 7 contributors for this article] (2021). Analysing indoor mycobiomes through a large‐scale citizen science study in Norway. Molecular Ecology. ISSN 0962-1083. 30, p. 2689–2705. doi: 10.1111/mec.15916. Show summary

  In the built environment, fungi can cause important deterioration of building materials and adverse health effects on occupants. Increased knowledge about indoor mycobiomes from different regions of the world, and their main environmental determinants, will enable improved indoor air quality management and identification of health risks. This is the first citizen science study about indoor mycobiomes at a large geographical scale in Europe, including 271 houses from Norway and 807 dust samples from three house compartments: outside of the building, living room and bathroom. The fungal community composition determined by DNA metabarcoding was clearly different between indoor and outdoor samples, but there were no significant differences between the two indoor compartments. The 32 selected variables, related to the outdoor environment, building features and occupant characteristics, accounted for 15% of the overall variation in community composition, with the house compartment as the key factor (7.6%). Next, the climate was the main driver of the dust mycobiomes (4.2%), while building and occupant variables had significant but minor influences (1.4% and 1.1%, respectively). The house‐dust mycobiomes were dominated by ascomycetes (⁓70%) with Capnodiales and Eurotiales as the most abundant orders. Compared to the outdoor samples, the indoor mycobiomes showed higher species richness, which is probably due to the mixture of fungi from outdoor and indoor sources. The main indoor indicator fungi belonged to two ecological groups with allergenic potential: xerophilic molds and skin‐associated yeasts. Our results suggest that citizen science is a successful approach for unraveling the built microbiome at large geographical scales.

• Morgado, Luis & Geml, József (2020). Modifications of Community Structure in Ectomycorrhizal Arctic Fungi as a Consequence of Global Warming. In Pérez-Moreno, Jesús; Guerin-Laguette, Alexis; Arzú, Roberto Flores & Yu, Fu-Qiang (Ed.), Mushrooms, Humans and Nature in a Changing World. Springer. ISSN 978-3-030-37377-1. p. 451–472. doi: 10.1007/978-3-030-37378-8_17.
• Ramírez-Cruz, Virginia; da Silva, Paula Santos; Villalobos-Arámbula, Alma Rosa; Matheny, P. Brandon; Noordeloos, Machiel E. & Morgado, Luis [Show all 8 contributors for this article] (2020). Two new species of Deconica (Agaricales, Basidiomycota) from Australia and Mexico. Mycological Progress. ISSN 1617-416X. 19(11), p. 1317–1328. doi: 10.1007/s11557-020-01629-w.
• Varga, Torda; Krizsán, Krisztina; Földi, Csenge; Dima, Bálint; Sánchez-García, Marisol & Sánchez-Ramírez, Santiago [Show all 62 contributors for this article] (2019). Megaphylogeny resolves global patterns of mushroom evolution. Nature Ecology and Evolution. ISSN 2397-334X. 3(4), p. 668–678. doi: 10.1038/s41559-019-0834-1.
• Zerrillo, L; Que, I; Vepris, O; Morgado, Luis Neves; Chan, A & Bierau, K [Show all 13 contributors for this article] (2019). pH-responsive poly(lactide-co-glycolide) nanoparticles containing near-infrared dye for visualization and hyaluronic acid for treatment of osteoarthritis. Journal of Controlled Release. ISSN 0168-3659. 309, p. 265–276. doi: 10.1016/j.jconrel.2019.07.031.
• Mundra, Sunil; Morgado, Luis Neves; Kjønaas, O. Janne & Kauserud, Håvard (2018). Soil depth matters: Bacteria, fungi and micro-eukaryotes are all strongly structured by soil depth. In Kettunen, Krista & Kiikkilä, Oili (Ed.), 3rd Conference on Ecology of Soil Microorganisms 2018. LUKE. ISSN 978-952-326-584-4. p. 157–157. Show summary

  The boreal forest is a key ecosystem for global C sequestration and storage. Microorganisms in soil have crucial functions in regulating these processes. Fungi are typically sharply structured with soil depth, but we largely lack such information for other microorganism, including bacteria and other micro-eukaryotes. To improve our knowledge of how different microorganisms are structured vertically and how they might interact, we investi-gated the communities of bacteria, fungi and micro-eukaryotes in four different soil horizons in natural birch forests in Western Norway. The communities of all three organismal groups were strongly structured along the vertical depth. Our results support the hypothesis that natural decrease in nutrient availability and pH differ-ences between organic and mineral horizons affect the distribution of soil microorganisms. Proteobacteria, Actinobacteria and Planctomycetes dominated in the uppermost organic layer while Acidobacteria and Firmicu-tes in mineral layers. Proportionally, fungi dominated in mineral layers whereas other micro-eukaryotes (Meta-zoa, Apicomplexa, Conosa, Ochrophyta and Chlorophyta) in organic layers. Ascomycota were relatively more abundant in mineral layers compared to Basidiomycota and Cryptomycota. Nematoda, Annelida and Arthropo-da showed decreasing trends with depth. Furthermore, different optima in the depth distribution of ectomy-corrhizal and saprotrophic genera was observed, supporting the view that different genera are adapted to different niches along the soil depth gradient. Network analyses will be used to infer tentative biotic interac-tions between the microbial groups and how this varies with soil depth.

• Geml, József; Morgado, Luis Neves; Semenova-Nelsen, Tatiana A. & Schilthuizen, Menno (2017). Changes in richness and community composition of ectomycorrhizal fungi among altitudinal vegetation types on Mount Kinabalu in Borneo. New Phytologist. ISSN 0028-646X. 215(1), p. 454–468. doi: 10.1111/nph.14566.
• Morgado, Luis Neves; Semenova, Tatiana A; Welker, Jeffrey M.; Walker, Marilyn D.; Smets, Erik & Geml, Jozsef (2016). Long-term increase in snow depth leads to compositional changes in arctic ectomycorrhizal fungal communities. Global Change Biology. ISSN 1354-1013. 22(9), p. 3080–3096. doi: 10.1111/gcb.13294.
• Morgado, Luis Neves; Noordeloos, Machiel E. & Hausknecht, Anton (2016). Clitopilus reticulosporus, a new species with unique spore ornamentation, its phylogenetic affinities and implications on the spore evolution theory. Mycological Progress. ISSN 1617-416X. 15:26(3). doi: 10.1007/s11557-016-1165-0.

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• Malicki, Brunon; Morgado, Luis; Nordén, Jenni; Markussen Bjorbækmo, Marit Frederikke; Skarpaas, Olav & Kjønaas, O. Janne [Show all 8 contributors for this article] (2022). Factors driving the diversity of eukaryotic microorganisms in boreal forest soils.
• Malicki, Brunon; Kauserud, Håvard; Morgado, Luis; Nordén, Björn; Nordén, Jenni & Bjorbækmo, Marit Frederikke Markussen (2021). Unearthing the factors driving eukaryotic diversity in boreal forest soils.
• Mundra, Sunil; Morgado, Luis Neves; Kauserud, Håvard & Kjønaas, O. Janne (2019). Compositional shift in communities of bacteria and fungi following tree species change from unmanaged birch to managed spruce forest.
• Mundra, Sunil; Morgado, Luis Neves; Kjønaas, O. Janne & Kauserud, Håvard (2018). Compositional shift in communities of bacteria and fungi following tree species change from unmanaged birch to managed spruce forest.
• Mundra, Sunil; Morgado, Luis Neves; Kjønaas, O. Janne & Kauserud, Håvard (2018). Soil depth matters: Bacteria, fungi and micro-eukaryotes are strongly structured by soil Depth.
• Mundra, Sunil; Morgado, Luis Neves; Kjønaas, O. Janne & Kauserud, Håvard (2018). Community of bacteria, fungi and micro-eukaryotes structured along soil forest stratification.
• Tonjer, Lea-Rebekka; Thoen, Ella; Morgado, Luis Neves; Mundra, Sunil; Nybakken, Line & Bryn, Anders [Show all 7 contributors for this article] (2018). Changes in the soil fungal community across the mountain birch tree line ecotone.
• Danielsen, Jørgen Skyrud; Kauserud, Håvard; Davey, Marie Louise; Mundra, Sunil & Morgado, Luis (2018). The establishment of spruce plantations in native birch forests causes major changes in the belowground fungal communities. Universitetet i Oslo.

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