Information about the microscope:
The Olympus FV 1000 is an inverted confocal microscope set up for live cell imaging and fixed samples. A confocal laser based microscope allows for higher signal to noise and better resolution imaging compared to widefield fluorescence imaging.
General specifications:
- Four laser-lines, 405, 473, 559, 635
- Four detectors for fluorescent light + a detector for transmitted light.
- Scan speed up to four frames per second, 512X512 pixels.
- Heating chamber set to 37°C
- CO2 controller
Objectives:
| Magnification | Type | Numerical Aperture | Working distance(mm) | Immersion |
|---|---|---|---|---|
| 20x | UplanApo | 0.7 | 0.65 | - |
| 40x | Semi-Apo | 0.85 | 0.2 | - |
| 60x | PlanApo | 1.35 | 0.13 | Oil |
| 100x | UPlanFLN | 1.4 | 0.2 | Oil |
More information on the objectives on the Olympus webpage