Abstract:
Outer membrane vesicles (OMVs) are a type of vesicles produced by the majority of Gram-negative bacteria. Thanks to the antigenic similarity between OMVs and the bacterial outer membrane, OMVs have proven to be promising candidates for the development of novel vaccines against bacterial pathogens.
Several protocols for OMV isolation and purification have been proposed and tested, but many lack the scalability, cost-effectiveness and reliability required for large-scale production of OMVs.
In the first part of this presentation we will report the optimization of a novel and easy to perform protocol for OMV concentration. The second part will instead be dedicated to describing the development and testing of an OMV-based vaccine against Gallibacterium anatis (App), a Gram-negative pathogen of great veterinary interest.